Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26803 | 5' | -58.6 | NC_005808.1 | + | 31944 | 0.67 | 0.386343 |
Target: 5'- cGGCGGCGCGUuucaCGCCGcaGGUGa---- -3' miRNA: 3'- cCCGUCGCGCA----GCGGCa-CCACcuaag -5' |
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26803 | 5' | -58.6 | NC_005808.1 | + | 41801 | 0.66 | 0.442209 |
Target: 5'- gGGcGCGGCGCGUCGCCGg--------- -3' miRNA: 3'- -CC-CGUCGCGCAGCGGCaccaccuaag -5' |
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26803 | 5' | -58.6 | NC_005808.1 | + | 4105 | 0.66 | 0.460831 |
Target: 5'- uGGGCGGCGCugaauucGUCGCgGUcGUcGGcUUCg -3' miRNA: 3'- -CCCGUCGCG-------CAGCGgCAcCA-CCuAAG- -5' |
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26803 | 5' | -58.6 | NC_005808.1 | + | 27791 | 0.66 | 0.461823 |
Target: 5'- aGGCAGCGC--CGCCGaGGUGcaGGUUUc -3' miRNA: 3'- cCCGUCGCGcaGCGGCaCCAC--CUAAG- -5' |
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26803 | 5' | -58.6 | NC_005808.1 | + | 29027 | 0.66 | 0.461823 |
Target: 5'- uGGCgAGCGCGcguucaacaUCGCCGUGGgcaccGAgUCa -3' miRNA: 3'- cCCG-UCGCGC---------AGCGGCACCac---CUaAG- -5' |
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26803 | 5' | -58.6 | NC_005808.1 | + | 38906 | 1.03 | 0.000943 |
Target: 5'- gGGGCAGCGCGUCGCCG-GGUGGAUUCg -3' miRNA: 3'- -CCCGUCGCGCAGCGGCaCCACCUAAG- -5' |
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26803 | 5' | -58.6 | NC_005808.1 | + | 3821 | 0.75 | 0.130587 |
Target: 5'- uGGGCAgGCGC-UUGCCGUGGUGag-UCa -3' miRNA: 3'- -CCCGU-CGCGcAGCGGCACCACcuaAG- -5' |
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26803 | 5' | -58.6 | NC_005808.1 | + | 5472 | 0.72 | 0.19124 |
Target: 5'- cGGGCGGCGagguGUUGCCGaUGGgGGGUa- -3' miRNA: 3'- -CCCGUCGCg---CAGCGGC-ACCaCCUAag -5' |
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26803 | 5' | -58.6 | NC_005808.1 | + | 13926 | 0.7 | 0.248599 |
Target: 5'- aGGGCGauGuCGCGcagCGCCGUGGacUGGAUg- -3' miRNA: 3'- -CCCGU--C-GCGCa--GCGGCACC--ACCUAag -5' |
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26803 | 5' | -58.6 | NC_005808.1 | + | 25090 | 0.7 | 0.282274 |
Target: 5'- -cGCAGCGCGcccggCGCCGUGGgauacagcGGGUg- -3' miRNA: 3'- ccCGUCGCGCa----GCGGCACCa-------CCUAag -5' |
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26803 | 5' | -58.6 | NC_005808.1 | + | 4539 | 0.67 | 0.404445 |
Target: 5'- uGGGCGGuCGUGcCGCCGcGGaacUGGAc-- -3' miRNA: 3'- -CCCGUC-GCGCaGCGGCaCC---ACCUaag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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