Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26805 | 3' | -55.3 | NC_005808.1 | + | 41015 | 0.66 | 0.602075 |
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Target: 5'- aCCAGGAaGGcGGCGGCcACUacAAGGAUa -3' miRNA: 3'- -GGUCCUaCC-UCGUCG-UGAccUUCCUG- -5' |
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| 26805 | 3' | -55.3 | NC_005808.1 | + | 13736 | 0.67 | 0.568473 |
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Target: 5'- gCgAGGAcgUGGcGCGGCGCUGGGAcaGCu -3' miRNA: 3'- -GgUCCU--ACCuCGUCGUGACCUUccUG- -5' |
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| 26805 | 3' | -55.3 | NC_005808.1 | + | 12718 | 0.67 | 0.556262 |
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Target: 5'- gCCGGGGggGGGGUGGUGCUagacccguucgcaGGAAGcGGCa -3' miRNA: 3'- -GGUCCUa-CCUCGUCGUGA-------------CCUUC-CUG- -5' |
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| 26805 | 3' | -55.3 | NC_005808.1 | + | 4340 | 0.72 | 0.284403 |
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Target: 5'- gCGGGcUGGGGUAGCAgaGGAAucggccGGACg -3' miRNA: 3'- gGUCCuACCUCGUCGUgaCCUU------CCUG- -5' |
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| 26805 | 3' | -55.3 | NC_005808.1 | + | 1488 | 0.73 | 0.256192 |
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Target: 5'- gCCGGGGUaucGCAGUGCUGGucGGACa -3' miRNA: 3'- -GGUCCUAccuCGUCGUGACCuuCCUG- -5' |
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| 26805 | 3' | -55.3 | NC_005808.1 | + | 39563 | 1.13 | 0.000356 |
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Target: 5'- aCCAGGAUGGAGCAGCACUGGAAGGACg -3' miRNA: 3'- -GGUCCUACCUCGUCGUGACCUUCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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