Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26805 | 5' | -63.5 | NC_005808.1 | + | 40616 | 0.66 | 0.273514 |
Target: 5'- -cGACCuCG-CGCGCCUacagCGugccuUCCCCCa -3' miRNA: 3'- uaCUGG-GCgGCGCGGAa---GC-----AGGGGGc -5' |
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26805 | 5' | -63.5 | NC_005808.1 | + | 21885 | 0.66 | 0.273514 |
Target: 5'- -cGGCCUGauggggugcCCGUGCCUcgUCGUCCagCCCa -3' miRNA: 3'- uaCUGGGC---------GGCGCGGA--AGCAGG--GGGc -5' |
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26805 | 5' | -63.5 | NC_005808.1 | + | 20787 | 0.66 | 0.26622 |
Target: 5'- --aACCCGCCGCugcaacuGCCggUGUCCgCCa -3' miRNA: 3'- uacUGGGCGGCG-------CGGaaGCAGGgGGc -5' |
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26805 | 5' | -63.5 | NC_005808.1 | + | 32115 | 0.66 | 0.247747 |
Target: 5'- --cGCCCugGCCGCuGCCgaccUCGacgCCCCCGa -3' miRNA: 3'- uacUGGG--CGGCG-CGGa---AGCa--GGGGGC- -5' |
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26805 | 5' | -63.5 | NC_005808.1 | + | 34023 | 0.67 | 0.241627 |
Target: 5'- cGUGACCgucgUGUCGCGCCUgUCGcugCCCgCCa -3' miRNA: 3'- -UACUGG----GCGGCGCGGA-AGCa--GGG-GGc -5' |
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26805 | 5' | -63.5 | NC_005808.1 | + | 1760 | 0.67 | 0.224022 |
Target: 5'- cAUGGCCgGCCuGCGCCUggcgCGUCgggCCGa -3' miRNA: 3'- -UACUGGgCGG-CGCGGAa---GCAGgg-GGC- -5' |
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26805 | 5' | -63.5 | NC_005808.1 | + | 13537 | 0.68 | 0.192076 |
Target: 5'- gAUGACCCGCUGaugGCCUaCGagCCgCCGg -3' miRNA: 3'- -UACUGGGCGGCg--CGGAaGCa-GGgGGC- -5' |
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26805 | 5' | -63.5 | NC_005808.1 | + | 11835 | 0.69 | 0.167691 |
Target: 5'- -aGACCgGCCGCGCCgcggUUGgccugggcgaaCCCCGc -3' miRNA: 3'- uaCUGGgCGGCGCGGa---AGCag---------GGGGC- -5' |
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26805 | 5' | -63.5 | NC_005808.1 | + | 25308 | 0.69 | 0.155741 |
Target: 5'- gAUGcgcGCCCGCgGCGCCggcuUCGUggacggcaauaUCCCCGg -3' miRNA: 3'- -UAC---UGGGCGgCGCGGa---AGCA-----------GGGGGC- -5' |
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26805 | 5' | -63.5 | NC_005808.1 | + | 13923 | 0.7 | 0.132697 |
Target: 5'- cGUGGCgCGCCGCGCUggCGaggCCgCCGg -3' miRNA: 3'- -UACUGgGCGGCGCGGaaGCa--GGgGGC- -5' |
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26805 | 5' | -63.5 | NC_005808.1 | + | 13130 | 0.71 | 0.115938 |
Target: 5'- --aACUgGCCGCGCgCUUCGUCgCCUGg -3' miRNA: 3'- uacUGGgCGGCGCG-GAAGCAGgGGGC- -5' |
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26805 | 5' | -63.5 | NC_005808.1 | + | 33276 | 0.72 | 0.090653 |
Target: 5'- --cGCCCGCCGCGCCgaCGUgCgCCGc -3' miRNA: 3'- uacUGGGCGGCGCGGaaGCAgGgGGC- -5' |
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26805 | 5' | -63.5 | NC_005808.1 | + | 39597 | 1.05 | 0.000228 |
Target: 5'- uAUGACCCGCCGCGCCUUCGUCCCCCGu -3' miRNA: 3'- -UACUGGGCGGCGCGGAAGCAGGGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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