Results 1 - 20 of 95 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26806 | 3' | -62.4 | NC_005808.1 | + | 35357 | 0.66 | 0.310833 |
Target: 5'- --uGGGCGGCcgacaucgccGAGgGUGaGCGCgGCGCa -3' miRNA: 3'- uguCCCGCCG----------UUCgCGCgCGUG-CGCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 31106 | 0.66 | 0.303398 |
Target: 5'- uGCAGGcCGGCGAGUG-GUGCGagauaGCGg -3' miRNA: 3'- -UGUCCcGCCGUUCGCgCGCGUg----CGCg -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 31022 | 0.66 | 0.303398 |
Target: 5'- --cGGGCuugccggccgaGGCAAucGCGCGgGCcaACGUGCg -3' miRNA: 3'- uguCCCG-----------CCGUU--CGCGCgCG--UGCGCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 15774 | 0.66 | 0.303398 |
Target: 5'- uCGGGGCGGCGcAGCcUGCcaaaaacaGCACGCc- -3' miRNA: 3'- uGUCCCGCCGU-UCGcGCG--------CGUGCGcg -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 21412 | 0.66 | 0.303398 |
Target: 5'- cGCAGaccaGCaGCAGGC-CGCGCAgcaggcCGCGCu -3' miRNA: 3'- -UGUCc---CGcCGUUCGcGCGCGU------GCGCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 8574 | 0.66 | 0.2961 |
Target: 5'- --uGGGCGucGUAGGcCGCGCGUGCG-GCc -3' miRNA: 3'- uguCCCGC--CGUUC-GCGCGCGUGCgCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 14412 | 0.66 | 0.2961 |
Target: 5'- aGCuGGGCGucgcCAGGCGCGCcugcgGCAcCGUGUu -3' miRNA: 3'- -UGuCCCGCc---GUUCGCGCG-----CGU-GCGCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 29567 | 0.66 | 0.2961 |
Target: 5'- uCGGGGUaucgcgucaGGUcGGC-CGCGCccagGCGCGCg -3' miRNA: 3'- uGUCCCG---------CCGuUCGcGCGCG----UGCGCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 19856 | 0.66 | 0.2961 |
Target: 5'- aGCAGcGUGGUGAGUucggGCGUGCcgaaggcgucGCGCGCc -3' miRNA: 3'- -UGUCcCGCCGUUCG----CGCGCG----------UGCGCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 25170 | 0.66 | 0.2961 |
Target: 5'- gGCGGGGCcacugcucaagGGCGA-CGUGgGCGC-CGCu -3' miRNA: 3'- -UGUCCCG-----------CCGUUcGCGCgCGUGcGCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 28756 | 0.66 | 0.28894 |
Target: 5'- cCAGcGCGGUGcgcuucucGGCGCGCacgGCGuCGCGCu -3' miRNA: 3'- uGUCcCGCCGU--------UCGCGCG---CGU-GCGCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 27770 | 0.66 | 0.284709 |
Target: 5'- --cGucCGGCucggacacguacacgAAGCGCGCGC-CGCGCa -3' miRNA: 3'- uguCccGCCG---------------UUCGCGCGCGuGCGCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 10297 | 0.66 | 0.281916 |
Target: 5'- ---uGGCGGCGaugaGGU-CGCGCaACGCGCu -3' miRNA: 3'- ugucCCGCCGU----UCGcGCGCG-UGCGCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 33377 | 0.66 | 0.281916 |
Target: 5'- cGCcGGGCGcGCcgcaGAaCGUGCGC-CGCGCc -3' miRNA: 3'- -UGuCCCGC-CG----UUcGCGCGCGuGCGCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 10500 | 0.66 | 0.281916 |
Target: 5'- cCAGGGCGuugaGCAAcGCGgcCGUGUugGCGg -3' miRNA: 3'- uGUCCCGC----CGUU-CGC--GCGCGugCGCg -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 6622 | 0.66 | 0.275028 |
Target: 5'- aGCGGGcgcGUGGCGcuGCGCGCGgACaCGUa -3' miRNA: 3'- -UGUCC---CGCCGUu-CGCGCGCgUGcGCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 20184 | 0.66 | 0.275028 |
Target: 5'- -aAGGcGUGGCuGGCGaaCGuCACGCGCu -3' miRNA: 3'- ugUCC-CGCCGuUCGCgcGC-GUGCGCG- -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 7065 | 0.66 | 0.275028 |
Target: 5'- --cGGGCGGC-AGCGuCGUuugaacagGCugGCGg -3' miRNA: 3'- uguCCCGCCGuUCGC-GCG--------CGugCGCg -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 10731 | 0.66 | 0.274347 |
Target: 5'- -gGGGGUcuugcacGGCGAGgGCGUGC-CGCa- -3' miRNA: 3'- ugUCCCG-------CCGUUCgCGCGCGuGCGcg -5' |
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26806 | 3' | -62.4 | NC_005808.1 | + | 9316 | 0.66 | 0.268276 |
Target: 5'- gACcuGGCGGCGcacgucGGCGCG-GCGgGCGg -3' miRNA: 3'- -UGucCCGCCGU------UCGCGCgCGUgCGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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