Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26806 | 5' | -52.9 | NC_005808.1 | + | 14582 | 0.66 | 0.761731 |
Target: 5'- -gGCugAUGCCGGcGGccGAuCCGCUUUu -3' miRNA: 3'- agCGugUACGGUU-CCaaCU-GGCGAAG- -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 41617 | 0.66 | 0.761731 |
Target: 5'- -gGCACcgGCCAGGcGgccaagUGGCUGCa-- -3' miRNA: 3'- agCGUGuaCGGUUC-Ca-----ACUGGCGaag -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 28787 | 0.66 | 0.717885 |
Target: 5'- gUCGCGCuUGCCcuGGUUG-CCGa-UCu -3' miRNA: 3'- -AGCGUGuACGGuuCCAACuGGCgaAG- -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 35507 | 0.66 | 0.717885 |
Target: 5'- gUGCGCGUgggcgcGCCGuGGGUaGGCCGCggcgUCa -3' miRNA: 3'- aGCGUGUA------CGGU-UCCAaCUGGCGa---AG- -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 9804 | 0.68 | 0.649579 |
Target: 5'- aCGCGCAgcaccagcgaggUGCCGAGGgcGgacuucGCCGcCUUCu -3' miRNA: 3'- aGCGUGU------------ACGGUUCCaaC------UGGC-GAAG- -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 19479 | 0.68 | 0.638062 |
Target: 5'- cUUGCGCcagGUGCCGguGGGcaGGCCGCUg- -3' miRNA: 3'- -AGCGUG---UACGGU--UCCaaCUGGCGAag -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 42110 | 0.68 | 0.638062 |
Target: 5'- -gGCGCAguaugcUGCCGAGGcauccGACCGCg-- -3' miRNA: 3'- agCGUGU------ACGGUUCCaa---CUGGCGaag -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 42374 | 0.68 | 0.638062 |
Target: 5'- gCGCGCcaAUGCCGAGGUgcgccaguucGCCGUgUCg -3' miRNA: 3'- aGCGUG--UACGGUUCCAac--------UGGCGaAG- -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 24706 | 0.68 | 0.615021 |
Target: 5'- gUC-CAUcgGUGCCAGucGGUcgGGCCGCUUCc -3' miRNA: 3'- -AGcGUG--UACGGUU--CCAa-CUGGCGAAG- -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 19870 | 0.68 | 0.611569 |
Target: 5'- uUCGgGCGUGCCgAAGGcgucgcgcgccuucUUGGCCGUggCa -3' miRNA: 3'- -AGCgUGUACGG-UUCC--------------AACUGGCGaaG- -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 21212 | 0.69 | 0.576047 |
Target: 5'- cUCGCGCAggcccagcgcGCCAuugcgacgaacucGGUUGAUCGUUUCg -3' miRNA: 3'- -AGCGUGUa---------CGGUu------------CCAACUGGCGAAG- -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 6433 | 0.69 | 0.546615 |
Target: 5'- -gGCGCcUGUCAcGGUcuUGGCUGCUUCc -3' miRNA: 3'- agCGUGuACGGUuCCA--ACUGGCGAAG- -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 16944 | 0.7 | 0.485082 |
Target: 5'- aCGUACAugacgccggcagaacUGCCGAGGUgGACgCGCUg- -3' miRNA: 3'- aGCGUGU---------------ACGGUUCCAaCUG-GCGAag -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 33320 | 0.72 | 0.41811 |
Target: 5'- aUGCGCA-GCCGguguaccGGGccGACCGCUUCc -3' miRNA: 3'- aGCGUGUaCGGU-------UCCaaCUGGCGAAG- -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 22545 | 0.74 | 0.31863 |
Target: 5'- gCGCGCGUGCCGguguugucgAGGaUGuuguuguGCCGCUUCu -3' miRNA: 3'- aGCGUGUACGGU---------UCCaAC-------UGGCGAAG- -5' |
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26806 | 5' | -52.9 | NC_005808.1 | + | 40050 | 1.11 | 0.000755 |
Target: 5'- gUCGCACAUGCCAAGGUUGACCGCUUCa -3' miRNA: 3'- -AGCGUGUACGGUUCCAACUGGCGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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