Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26808 | 5' | -59.5 | NC_005808.1 | + | 5541 | 0.66 | 0.421318 |
Target: 5'- aGGUUCCGCccagg-UCGCCCGCCaCGGu -3' miRNA: 3'- -UUAGGGCGacgacaAGCGGGUGG-GCC- -5' |
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26808 | 5' | -59.5 | NC_005808.1 | + | 20220 | 0.66 | 0.417558 |
Target: 5'- --gCCCGCggccgGCUGUUgGCCUugggugccuugcuCCUGGg -3' miRNA: 3'- uuaGGGCGa----CGACAAgCGGGu------------GGGCC- -5' |
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26808 | 5' | -59.5 | NC_005808.1 | + | 14863 | 0.66 | 0.411027 |
Target: 5'- ---gCCGCacGCUGggCGCCUuuaaguuGCCCGGc -3' miRNA: 3'- uuagGGCGa-CGACaaGCGGG-------UGGGCC- -5' |
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26808 | 5' | -59.5 | NC_005808.1 | + | 33051 | 0.66 | 0.393625 |
Target: 5'- -uUCCCGCgUGCUG-UCGUgCGCUucauCGGg -3' miRNA: 3'- uuAGGGCG-ACGACaAGCGgGUGG----GCC- -5' |
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26808 | 5' | -59.5 | NC_005808.1 | + | 38553 | 0.67 | 0.36714 |
Target: 5'- aAGUUCCGCccgGCUGcgcUCGaCCCGCgCGGc -3' miRNA: 3'- -UUAGGGCGa--CGACa--AGC-GGGUGgGCC- -5' |
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26808 | 5' | -59.5 | NC_005808.1 | + | 14934 | 0.67 | 0.350176 |
Target: 5'- --aCCaCGgUGgaGguggCGCCCACCUGGg -3' miRNA: 3'- uuaGG-GCgACgaCaa--GCGGGUGGGCC- -5' |
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26808 | 5' | -59.5 | NC_005808.1 | + | 12731 | 0.67 | 0.341906 |
Target: 5'- --gCCCGUuggUGCUGggUGCCgCGCCCa- -3' miRNA: 3'- uuaGGGCG---ACGACaaGCGG-GUGGGcc -5' |
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26808 | 5' | -59.5 | NC_005808.1 | + | 19417 | 0.67 | 0.341906 |
Target: 5'- --aCCUGCUGCUG-UCGCaCCAgCaGGg -3' miRNA: 3'- uuaGGGCGACGACaAGCG-GGUgGgCC- -5' |
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26808 | 5' | -59.5 | NC_005808.1 | + | 25120 | 0.68 | 0.310249 |
Target: 5'- -uUCUCGCcGCUGUUCGUgCAggcCCUGGc -3' miRNA: 3'- uuAGGGCGaCGACAAGCGgGU---GGGCC- -5' |
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26808 | 5' | -59.5 | NC_005808.1 | + | 18290 | 0.69 | 0.280873 |
Target: 5'- --aCCCGCgGCUGcUCGCCgACCUc- -3' miRNA: 3'- uuaGGGCGaCGACaAGCGGgUGGGcc -5' |
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26808 | 5' | -59.5 | NC_005808.1 | + | 40702 | 1.07 | 0.000382 |
Target: 5'- aAAUCCCGCUGCUGUUCGCCCACCCGGc -3' miRNA: 3'- -UUAGGGCGACGACAAGCGGGUGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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