Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26809 | 5' | -59.4 | NC_005808.1 | + | 35182 | 0.66 | 0.429035 |
Target: 5'- gGCCGGCCAUccGgUGCCCGGc-UUCAa -3' miRNA: 3'- -CGGCCGGUAc-UgGCGGGCCacAAGUa -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 12695 | 0.66 | 0.410219 |
Target: 5'- gGCCcGCCuUGAuaGCCUGGcUGUUCAa -3' miRNA: 3'- -CGGcCGGuACUggCGGGCC-ACAAGUa -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 41285 | 0.66 | 0.409292 |
Target: 5'- uGUCGGCCugGUGGCCGUCgcccugggcgcggCGGUGUgggCGg -3' miRNA: 3'- -CGGCCGG--UACUGGCGG-------------GCCACAa--GUa -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 4431 | 0.66 | 0.401005 |
Target: 5'- uGCCGcGCCAgggGgaaGCCGCCagCGGUGgcaccgUCAUg -3' miRNA: 3'- -CGGC-CGGUa--C---UGGCGG--GCCACa-----AGUA- -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 26514 | 0.66 | 0.391925 |
Target: 5'- aGCCGGCaucauucACCGCCaCGGUuUUCAg -3' miRNA: 3'- -CGGCCGguac---UGGCGG-GCCAcAAGUa -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 13979 | 0.66 | 0.391925 |
Target: 5'- gGCCGGCCucaucCgCGCCCGGU--UCAa -3' miRNA: 3'- -CGGCCGGuacu-G-GCGGGCCAcaAGUa -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 2660 | 0.67 | 0.382979 |
Target: 5'- --gGGCCAgcgG-UCGCCCaGGUGUUCGa -3' miRNA: 3'- cggCCGGUa--CuGGCGGG-CCACAAGUa -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 40360 | 0.67 | 0.37417 |
Target: 5'- uGCCGGCCAag---GCCCGGcGUUUAUa -3' miRNA: 3'- -CGGCCGGUacuggCGGGCCaCAAGUA- -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 30584 | 0.67 | 0.37417 |
Target: 5'- cGCCGGCCAcGGCCGCgCUGcUGgcCGa -3' miRNA: 3'- -CGGCCGGUaCUGGCG-GGCcACaaGUa -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 8986 | 0.67 | 0.348578 |
Target: 5'- cGgUGGCCGUG-CCGCCagCGGUGgcCGUg -3' miRNA: 3'- -CgGCCGGUACuGGCGG--GCCACaaGUA- -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 6396 | 0.68 | 0.324259 |
Target: 5'- gGCCGGCCG-----GCCCGGUGUagUCGc -3' miRNA: 3'- -CGGCCGGUacuggCGGGCCACA--AGUa -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 694 | 0.68 | 0.301226 |
Target: 5'- cGCCGGCCG-GGCCGCCCa-------- -3' miRNA: 3'- -CGGCCGGUaCUGGCGGGccacaagua -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 34831 | 0.7 | 0.215975 |
Target: 5'- cGCCGGCgCA-GAUCGCgCCGGUGcugCAa -3' miRNA: 3'- -CGGCCG-GUaCUGGCG-GGCCACaa-GUa -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 24576 | 0.73 | 0.152251 |
Target: 5'- gGCCGGCCAcggucgGGCCGUCCGGcGUg--- -3' miRNA: 3'- -CGGCCGGUa-----CUGGCGGGCCaCAagua -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 41573 | 0.83 | 0.026467 |
Target: 5'- cGCCGGCCGUGGCCuUCCaGGUGUUCGa -3' miRNA: 3'- -CGGCCGGUACUGGcGGG-CCACAAGUa -5' |
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26809 | 5' | -59.4 | NC_005808.1 | + | 40853 | 1.06 | 0.000414 |
Target: 5'- gGCCGGCCAUGACCGCCCGGUGUUCAUc -3' miRNA: 3'- -CGGCCGGUACUGGCGGGCCACAAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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