Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26812 | 3' | -55.1 | NC_005808.1 | + | 10933 | 0.66 | 0.686862 |
Target: 5'- uGCUuguGGCCggUGUaGGCCCaccaaGCGGCa -3' miRNA: 3'- gUGAuu-CUGGuuGCA-CCGGG-----CGCUGc -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 31043 | 0.66 | 0.685758 |
Target: 5'- uCGCgcGGGCCAACGUgcgcgaaGGCCCGa-ACa -3' miRNA: 3'- -GUGauUCUGGUUGCA-------CCGGGCgcUGc -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 10171 | 0.66 | 0.653546 |
Target: 5'- uCGCUGccgucgucuuGGCaGACGUGGCgCGCGGCu -3' miRNA: 3'- -GUGAUu---------CUGgUUGCACCGgGCGCUGc -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 33179 | 0.66 | 0.642384 |
Target: 5'- aCGCUAuucaAGACCuGCaa-GCCCGCGGCc -3' miRNA: 3'- -GUGAU----UCUGGuUGcacCGGGCGCUGc -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 24461 | 0.66 | 0.642384 |
Target: 5'- aCAUUGAcGCCGACGaccugcugcuUGGCCUGCGcCa -3' miRNA: 3'- -GUGAUUcUGGUUGC----------ACCGGGCGCuGc -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 13237 | 0.66 | 0.635682 |
Target: 5'- gGCUGGGgcaaggccACCGGCGcgugguucgaggacuUGGCCgGCGACc -3' miRNA: 3'- gUGAUUC--------UGGUUGC---------------ACCGGgCGCUGc -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 36180 | 0.66 | 0.631214 |
Target: 5'- gAC-AGGcGCCAACGacaUGGgCCGCGACu -3' miRNA: 3'- gUGaUUC-UGGUUGC---ACCgGGCGCUGc -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 16047 | 0.66 | 0.631214 |
Target: 5'- gUugU-AGGCCAguucgcgcACGUGGCCgccgcgCGCGGCGc -3' miRNA: 3'- -GugAuUCUGGU--------UGCACCGG------GCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 25498 | 0.67 | 0.620044 |
Target: 5'- uGCUuGG-UCAGgGUcaGGCCCGCGAUGa -3' miRNA: 3'- gUGAuUCuGGUUgCA--CCGGGCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 37022 | 0.67 | 0.608885 |
Target: 5'- aCACgguggcGGCCAGCGuUGGCgccgucgauaCCGUGGCGg -3' miRNA: 3'- -GUGauu---CUGGUUGC-ACCG----------GGCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 13692 | 0.67 | 0.597747 |
Target: 5'- uUugUGGGACga--GUGGUCgGCGACGg -3' miRNA: 3'- -GugAUUCUGguugCACCGGgCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 12006 | 0.67 | 0.597747 |
Target: 5'- cCACgucGGCCAGCagcgcggccGUGGCCgGCGAgGu -3' miRNA: 3'- -GUGauuCUGGUUG---------CACCGGgCGCUgC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 31905 | 0.67 | 0.586638 |
Target: 5'- cCAUcGAGGCCGA-GU-GCCUGCGGCGu -3' miRNA: 3'- -GUGaUUCUGGUUgCAcCGGGCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 37717 | 0.67 | 0.586638 |
Target: 5'- ---aAGGACgCAugauuGCGUGGCUCGcCGGCGa -3' miRNA: 3'- gugaUUCUG-GU-----UGCACCGGGC-GCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 6617 | 0.67 | 0.57557 |
Target: 5'- -----uGACCAGCG-GGCgCGUGGCGc -3' miRNA: 3'- gugauuCUGGUUGCaCCGgGCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 40441 | 0.67 | 0.57557 |
Target: 5'- aACUGuucGCCAACGUGuaCgGCGGCGc -3' miRNA: 3'- gUGAUuc-UGGUUGCACcgGgCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 1707 | 0.68 | 0.564549 |
Target: 5'- uCGCcguGGGCCGGCGU-GCCgGCGAgGg -3' miRNA: 3'- -GUGau-UCUGGUUGCAcCGGgCGCUgC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 7377 | 0.68 | 0.531855 |
Target: 5'- -----cGuCCAGCGgcgGGCaCCGCGGCGa -3' miRNA: 3'- gugauuCuGGUUGCa--CCG-GGCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 12626 | 0.68 | 0.531855 |
Target: 5'- gGCUGcauccAGGCCGuCGU-GCgCCGCGACGa -3' miRNA: 3'- gUGAU-----UCUGGUuGCAcCG-GGCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 18879 | 0.68 | 0.521105 |
Target: 5'- cCACcGAGGC--AC-UGGCCCGCGACc -3' miRNA: 3'- -GUGaUUCUGguUGcACCGGGCGCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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