Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26812 | 3' | -55.1 | NC_005808.1 | + | 10171 | 0.66 | 0.653546 |
Target: 5'- uCGCUGccgucgucuuGGCaGACGUGGCgCGCGGCu -3' miRNA: 3'- -GUGAUu---------CUGgUUGCACCGgGCGCUGc -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 7377 | 0.68 | 0.531855 |
Target: 5'- -----cGuCCAGCGgcgGGCaCCGCGGCGa -3' miRNA: 3'- gugauuCuGGUUGCa--CCG-GGCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 12626 | 0.68 | 0.531855 |
Target: 5'- gGCUGcauccAGGCCGuCGU-GCgCCGCGACGa -3' miRNA: 3'- gUGAU-----UCUGGUuGCAcCG-GGCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 33750 | 0.8 | 0.100059 |
Target: 5'- gACcGAGGCCAACGcgcUGGCCCGCGuuGCGg -3' miRNA: 3'- gUGaUUCUGGUUGC---ACCGGGCGC--UGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 17918 | 0.79 | 0.109063 |
Target: 5'- aGCcuGGGCgugGACGUGGCCCGCGGCGg -3' miRNA: 3'- gUGauUCUGg--UUGCACCGGGCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 14022 | 0.79 | 0.122253 |
Target: 5'- aCGCUGccgguGGCCGACGUGcGCgCCGCGAUGg -3' miRNA: 3'- -GUGAUu----CUGGUUGCAC-CG-GGCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 15146 | 0.72 | 0.330422 |
Target: 5'- gCGCUGGcauACCAGCGccagGGCCUGCGGCc -3' miRNA: 3'- -GUGAUUc--UGGUUGCa---CCGGGCGCUGc -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 23903 | 0.69 | 0.458618 |
Target: 5'- gCGCUGcuGCUGACcgaGGCCCGCGugGu -3' miRNA: 3'- -GUGAUucUGGUUGca-CCGGGCGCugC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 12449 | 0.69 | 0.489396 |
Target: 5'- cCGCUGAuGuCCGACGcggugcaGGCCgGCGGCGu -3' miRNA: 3'- -GUGAUU-CuGGUUGCa------CCGGgCGCUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 22967 | 0.69 | 0.499869 |
Target: 5'- aCGCU--GGCCGAUGUcgcGGCCCGCaugaccccgGACGg -3' miRNA: 3'- -GUGAuuCUGGUUGCA---CCGGGCG---------CUGC- -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 18879 | 0.68 | 0.521105 |
Target: 5'- cCACcGAGGC--AC-UGGCCCGCGACc -3' miRNA: 3'- -GUGaUUCUGguUGcACCGGGCGCUGc -5' |
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26812 | 3' | -55.1 | NC_005808.1 | + | 10933 | 0.66 | 0.686862 |
Target: 5'- uGCUuguGGCCggUGUaGGCCCaccaaGCGGCa -3' miRNA: 3'- gUGAuu-CUGGuuGCA-CCGGG-----CGCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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