miRNA: 3'- acuaCCGCC-CCuaCuGCGGCCGua--AG- -5'
miRNA: 3'- -ACuACCGCC-CCUACuGCGG---CCGUaag -5'
miRNA: 3'- aCUACCGCcCCuacuGCGGCCGuAAG- -5'
miRNA: 3'- -ACUa---CCGCcCCUACUgCGGCCG--UAAG- -5'
miRNA: 3'- -ACuaCCGCCCCUa-CUGCggCCGuaAG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26813 | 5' | -57.5 | NC_005808.1 | + | 29327 | 0.66 | 0.471613 |
|
Target: 5'- ---aGGCGGuGGcgGuCGCCGGCcaagUCc -3' miRNA: 3'- acuaCCGCC-CCuaCuGCGGCCGua--AG- -5' |
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| 26813 | 5' | -57.5 | NC_005808.1 | + | 15141 | 0.67 | 0.431808 |
|
Target: 5'- aUGcUGGCGGcGGAUG-CGCCcagGGCGa-- -3' miRNA: 3'- -ACuACCGCC-CCUACuGCGG---CCGUaag -5' |
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| 26813 | 5' | -57.5 | NC_005808.1 | + | 8638 | 0.69 | 0.349937 |
|
Target: 5'- cGGUGGCGaGGu---CGCCGGCcUUCa -3' miRNA: 3'- aCUACCGCcCCuacuGCGGCCGuAAG- -5' |
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| 26813 | 5' | -57.5 | NC_005808.1 | + | 28617 | 0.72 | 0.204138 |
|
Target: 5'- uUGAaccgGGCGcGGAUGAgGCCGGCcaGUUCc -3' miRNA: 3'- -ACUa---CCGCcCCUACUgCGGCCG--UAAG- -5' |
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| 26813 | 5' | -57.5 | NC_005808.1 | + | 23579 | 0.72 | 0.204138 |
|
Target: 5'- gUGcgGGCGGGGGgcGACGauGGCcgUCa -3' miRNA: 3'- -ACuaCCGCCCCUa-CUGCggCCGuaAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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