Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26814 | 5' | -56.6 | NC_005809.1 | + | 15326 | 0.77 | 0.120603 |
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Target: 5'- cGGCcaAgAGCCUgGGGCGCCGGCUgGAc -3' miRNA: 3'- -CCGa-UgUCGGAaCCCGUGGCUGAgCU- -5' |
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| 26814 | 5' | -56.6 | NC_005809.1 | + | 7148 | 0.75 | 0.156959 |
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Target: 5'- cGGCUGCGGCCUggaugaccagggccGGGCGCUugucgGAUUCGGg -3' miRNA: 3'- -CCGAUGUCGGAa-------------CCCGUGG-----CUGAGCU- -5' |
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| 26814 | 5' | -56.6 | NC_005809.1 | + | 41613 | 0.74 | 0.183088 |
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Target: 5'- uGGCUGCAagcuGCCgUGGGCACCGcGCagGAc -3' miRNA: 3'- -CCGAUGU----CGGaACCCGUGGC-UGagCU- -5' |
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| 26814 | 5' | -56.6 | NC_005809.1 | + | 38319 | 0.7 | 0.34141 |
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Target: 5'- cGGCgcugACGGCC---GGCACCGACUaCGc -3' miRNA: 3'- -CCGa---UGUCGGaacCCGUGGCUGA-GCu -5' |
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| 26814 | 5' | -56.6 | NC_005809.1 | + | 7448 | 0.7 | 0.34141 |
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Target: 5'- cGGCUuCGGCCUucgcgcgaaUGGccuuGCACCagGACUCGAc -3' miRNA: 3'- -CCGAuGUCGGA---------ACC----CGUGG--CUGAGCU- -5' |
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| 26814 | 5' | -56.6 | NC_005809.1 | + | 14590 | 0.69 | 0.38388 |
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Target: 5'- cGGCUACAGCCUgaUGGcccagccCACCGaggacguGCUCGc -3' miRNA: 3'- -CCGAUGUCGGA--ACCc------GUGGC-------UGAGCu -5' |
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| 26814 | 5' | -56.6 | NC_005809.1 | + | 9909 | 0.69 | 0.384782 |
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Target: 5'- gGGCUucacgucggGCAGCUUcGcGGCGCCGAacUUCGAc -3' miRNA: 3'- -CCGA---------UGUCGGAaC-CCGUGGCU--GAGCU- -5' |
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| 26814 | 5' | -56.6 | NC_005809.1 | + | 32253 | 0.66 | 0.57793 |
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Target: 5'- cGGCUACGGCCU--GGCgaaACUGGCgaagUGGc -3' miRNA: 3'- -CCGAUGUCGGAacCCG---UGGCUGa---GCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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