Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26819 | 3' | -57.7 | NC_005809.1 | + | 31305 | 0.66 | 0.562486 |
Target: 5'- gGCgacCGCGUGGUG--GCCCGGCu-GGa -3' miRNA: 3'- -CGac-GCGCACCACgaUGGGCUGcuCC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 1708 | 0.66 | 0.562486 |
Target: 5'- --cGC-CGUGGgccgGCgugCCGGCGAGGg -3' miRNA: 3'- cgaCGcGCACCa---CGaugGGCUGCUCC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 12064 | 0.66 | 0.562485 |
Target: 5'- aGCUGCGCcaccucGUGCUcaACCuCGACGAc- -3' miRNA: 3'- -CGACGCGcac---CACGA--UGG-GCUGCUcc -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 4811 | 0.66 | 0.551782 |
Target: 5'- cGCUGCGgGau-UGCUGgCCGGCGAcaGGu -3' miRNA: 3'- -CGACGCgCaccACGAUgGGCUGCU--CC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 31989 | 0.66 | 0.551781 |
Target: 5'- gGCUGCGCGccugGGUaCUACgCC--CGAGGc -3' miRNA: 3'- -CGACGCGCa---CCAcGAUG-GGcuGCUCC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 16828 | 0.66 | 0.54114 |
Target: 5'- aGCaGCGCGcccaGcGUGCUGCCgcCGGUGAGGa -3' miRNA: 3'- -CGaCGCGCa---C-CACGAUGG--GCUGCUCC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 4124 | 0.66 | 0.530569 |
Target: 5'- cGCUGCaGCGU--UGCUuCCCGGCGcauaguGGa -3' miRNA: 3'- -CGACG-CGCAccACGAuGGGCUGCu-----CC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 35482 | 0.66 | 0.530568 |
Target: 5'- -gUGCGCGUGG-GC-GCgCCGugGguAGGc -3' miRNA: 3'- cgACGCGCACCaCGaUG-GGCugC--UCC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 8170 | 0.66 | 0.520073 |
Target: 5'- cCUGCGCGUgcgcccGGaUGCUGCC-GGCGAu- -3' miRNA: 3'- cGACGCGCA------CC-ACGAUGGgCUGCUcc -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 15099 | 0.66 | 0.50966 |
Target: 5'- cCU-CGCGUGGUGCgUGCgCGGCGcgcuGGc -3' miRNA: 3'- cGAcGCGCACCACG-AUGgGCUGCu---CC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 20052 | 0.67 | 0.478973 |
Target: 5'- cGCUGCGCGUGcucGCUGCggggaUGAUGGcGGg -3' miRNA: 3'- -CGACGCGCACca-CGAUGg----GCUGCU-CC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 20687 | 0.67 | 0.477965 |
Target: 5'- -aUGCGCGcacucuaUGG-GCUGgaCGACGAGGc -3' miRNA: 3'- cgACGCGC-------ACCaCGAUggGCUGCUCC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 32036 | 0.67 | 0.459026 |
Target: 5'- cGUUGCGCGUGGcgGCggaaagcgUGgCCGGCGGu- -3' miRNA: 3'- -CGACGCGCACCa-CG--------AUgGGCUGCUcc -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 5469 | 0.67 | 0.449221 |
Target: 5'- cGCcGgGCGgcgaGGUGUUG-CCGAUGGGGg -3' miRNA: 3'- -CGaCgCGCa---CCACGAUgGGCUGCUCC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 6622 | 0.67 | 0.44922 |
Target: 5'- aGCggGCGCGUGGcGCUGCgCG-CGGa- -3' miRNA: 3'- -CGa-CGCGCACCaCGAUGgGCuGCUcc -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 1670 | 0.68 | 0.439531 |
Target: 5'- uUUGCGCGUGaUGCUGCC--GCGcAGGu -3' miRNA: 3'- cGACGCGCACcACGAUGGgcUGC-UCC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 12363 | 0.68 | 0.439531 |
Target: 5'- --aGUGCGUGcgcGUGCUcaaGCCCGGCGGc- -3' miRNA: 3'- cgaCGCGCAC---CACGA---UGGGCUGCUcc -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 14787 | 0.68 | 0.439531 |
Target: 5'- gGCUGCGCGgcgcgcgcuucgUGuacGUGUccgaGCCgGACGAGGg -3' miRNA: 3'- -CGACGCGC------------AC---CACGa---UGGgCUGCUCC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 16644 | 0.68 | 0.411198 |
Target: 5'- --gGUGCa-GGUGCcGgCCGACGAGGg -3' miRNA: 3'- cgaCGCGcaCCACGaUgGGCUGCUCC- -5' |
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26819 | 3' | -57.7 | NC_005809.1 | + | 16117 | 0.69 | 0.366584 |
Target: 5'- gGCUGCaCGUcGGUGgc-CCCGACGuAGGa -3' miRNA: 3'- -CGACGcGCA-CCACgauGGGCUGC-UCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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