Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26830 | 3' | -57.1 | NC_005809.1 | + | 22070 | 0.66 | 0.552169 |
Target: 5'- cGGGuCGCgGUCGGCGCGCGgC--UCGAu -3' miRNA: 3'- -CUC-GUGgCAGUCGUGCGUgGacAGCU- -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 26320 | 0.66 | 0.552169 |
Target: 5'- aGGCACgGUCuuGaCGCGCACCg--CGAa -3' miRNA: 3'- cUCGUGgCAGu-C-GUGCGUGGacaGCU- -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 13289 | 0.66 | 0.541315 |
Target: 5'- cAGCGCCGauugCAGCGCGCcgggGCCggcGUUGu -3' miRNA: 3'- cUCGUGGCa---GUCGUGCG----UGGa--CAGCu -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 25188 | 0.66 | 0.530534 |
Target: 5'- aAGCGgugCGUCGGCGCGaugcaggcaUACCUGUCGc -3' miRNA: 3'- cUCGUg--GCAGUCGUGC---------GUGGACAGCu -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 19738 | 0.66 | 0.530534 |
Target: 5'- -cGCGCUGUCGGCugucggcaGCGCGgCgaaGUCGGg -3' miRNA: 3'- cuCGUGGCAGUCG--------UGCGUgGa--CAGCU- -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 29089 | 0.66 | 0.530534 |
Target: 5'- aGGCGCgGUCGGCAuCGC-CCagGUCa- -3' miRNA: 3'- cUCGUGgCAGUCGU-GCGuGGa-CAGcu -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 38871 | 0.66 | 0.519832 |
Target: 5'- -uGCGCCGcauggggCAGCGCGuCGCCgugGUgGAu -3' miRNA: 3'- cuCGUGGCa------GUCGUGC-GUGGa--CAgCU- -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 9186 | 0.66 | 0.509215 |
Target: 5'- uGGCGCCGUCG--ACGUugGCCUGcUCGGc -3' miRNA: 3'- cUCGUGGCAGUcgUGCG--UGGAC-AGCU- -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 6703 | 0.66 | 0.498691 |
Target: 5'- aGGCACaccuuGUCcugGGCGCGCAUCUGcUUGAg -3' miRNA: 3'- cUCGUGg----CAG---UCGUGCGUGGAC-AGCU- -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 413 | 0.67 | 0.488265 |
Target: 5'- cAGCACggcggugauCGUCGGC-CGUACCuUGUCGu -3' miRNA: 3'- cUCGUG---------GCAGUCGuGCGUGG-ACAGCu -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 8663 | 0.67 | 0.488265 |
Target: 5'- -cGCGCgGUCGGCauugcgugccgcGCGCACCUcggcGUUGGu -3' miRNA: 3'- cuCGUGgCAGUCG------------UGCGUGGA----CAGCU- -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 14786 | 0.67 | 0.477943 |
Target: 5'- cGGCugCG-CGGCGCGCGCUucguguacgUGUcCGAg -3' miRNA: 3'- cUCGugGCaGUCGUGCGUGG---------ACA-GCU- -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 33995 | 0.67 | 0.477943 |
Target: 5'- -cGCcgugACCGUCGuGU-CGCGCCUGUCGc -3' miRNA: 3'- cuCG----UGGCAGU-CGuGCGUGGACAGCu -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 12617 | 0.67 | 0.467729 |
Target: 5'- uGGCGCCGacgcgcUCGGCAuCGCugCUGcCGu -3' miRNA: 3'- cUCGUGGC------AGUCGU-GCGugGACaGCu -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 3869 | 0.67 | 0.467729 |
Target: 5'- aGGCGCCGUCgcuguaGGcCGCGCugCcGUCGc -3' miRNA: 3'- cUCGUGGCAG------UC-GUGCGugGaCAGCu -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 27474 | 0.67 | 0.467729 |
Target: 5'- -cGCGCCG-C-GCACGCACCacgCGAg -3' miRNA: 3'- cuCGUGGCaGuCGUGCGUGGacaGCU- -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 23420 | 0.67 | 0.457629 |
Target: 5'- -cGCGCCgGUCAGUGCGaCGCCgGcCGGg -3' miRNA: 3'- cuCGUGG-CAGUCGUGC-GUGGaCaGCU- -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 33186 | 0.67 | 0.437786 |
Target: 5'- uGGGCGCCcUCGGCACGgcCACCgcuggCGAa -3' miRNA: 3'- -CUCGUGGcAGUCGUGC--GUGGaca--GCU- -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 37016 | 0.68 | 0.428052 |
Target: 5'- uGGCGCCGUCgauaccguGGCGgGCgACCUGggCGGa -3' miRNA: 3'- cUCGUGGCAG--------UCGUgCG-UGGACa-GCU- -5' |
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26830 | 3' | -57.1 | NC_005809.1 | + | 19479 | 0.68 | 0.422273 |
Target: 5'- uGGGCGCCGcCguuggcgucggggcgAGCAUGUACaUGUCGAa -3' miRNA: 3'- -CUCGUGGCaG---------------UCGUGCGUGgACAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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