miRNA: 3'- -UGUAGCU----GCG-------GCACCGA-UGGUUGCUC- -5'
miRNA: 3'- -UGUag-CUGCGGCaCCG-AUGGU----UGCUc -5'
miRNA: 3'- -UGU-AGCUG---CGGCACCG-----------A-------------UGGUUGCUC- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26831 | 3' | -54.6 | NC_005809.1 | + | 37685 | 0.76 | 0.202934 |
|
Target: 5'- aACAUCGAaggaCGCaugauugCGUGGCUcGCCGGCGAGg -3' miRNA: 3'- -UGUAGCU----GCG-------GCACCGA-UGGUUGCUC- -5' |
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| 26831 | 3' | -54.6 | NC_005809.1 | + | 39399 | 0.67 | 0.593157 |
|
Target: 5'- cGCAgcgGACGCCG-GGCuUGCCAugcuGCGAu -3' miRNA: 3'- -UGUag-CUGCGGCaCCG-AUGGU----UGCUc -5' |
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| 26831 | 3' | -54.6 | NC_005809.1 | + | 41855 | 0.68 | 0.580887 |
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Target: 5'- gGCGcUCGACuugGCCGUGGCcgacaagaucaUcaaggaaaacccaGCCAGCGAGg -3' miRNA: 3'- -UGU-AGCUG---CGGCACCG-----------A-------------UGGUUGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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