Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26840 | 3' | -60.7 | NC_005809.1 | + | 37964 | 0.66 | 0.356599 |
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Target: 5'- cCGCCgacaucauCGCCGaggcCGAGCAGUuccUCGCCUgGCGc -3' miRNA: 3'- -GCGG--------GUGGU----GCUCGUCA---AGCGGG-CGC- -5' |
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| 26840 | 3' | -60.7 | NC_005809.1 | + | 38052 | 0.71 | 0.186188 |
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Target: 5'- cCGCCCAgcacgccacguUCACGGGCGc--CGCCCGUGa -3' miRNA: 3'- -GCGGGU-----------GGUGCUCGUcaaGCGGGCGC- -5' |
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| 26840 | 3' | -60.7 | NC_005809.1 | + | 38839 | 0.66 | 0.399797 |
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Target: 5'- cCGCCCucaucaaauCCGCGAGCAugagcGgaUGCgCCGCa -3' miRNA: 3'- -GCGGGu--------GGUGCUCGU-----CaaGCG-GGCGc -5' |
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| 26840 | 3' | -60.7 | NC_005809.1 | + | 38844 | 0.67 | 0.3323 |
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Target: 5'- uGCCCACCACcGGCGugaacggcacGggCGCCacgaGCGc -3' miRNA: 3'- gCGGGUGGUGcUCGU----------CaaGCGGg---CGC- -5' |
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| 26840 | 3' | -60.7 | NC_005809.1 | + | 39752 | 0.67 | 0.340263 |
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Target: 5'- gCGCgUgGCCGCGAGCAccUgGCCCGaCGa -3' miRNA: 3'- -GCG-GgUGGUGCUCGUcaAgCGGGC-GC- -5' |
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| 26840 | 3' | -60.7 | NC_005809.1 | + | 40576 | 0.7 | 0.210544 |
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Target: 5'- gCGCCUgggccucaagaucaGCCACuGGCAGguggCGCCCGUa -3' miRNA: 3'- -GCGGG--------------UGGUGcUCGUCaa--GCGGGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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