Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26840 | 3' | -60.7 | NC_005809.1 | + | 4042 | 0.66 | 0.38212 |
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Target: 5'- cCGgCCGCgGCGAGCGGcagGCCaGCGg -3' miRNA: 3'- -GCgGGUGgUGCUCGUCaagCGGgCGC- -5' |
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| 26840 | 3' | -60.7 | NC_005809.1 | + | 3224 | 0.66 | 0.373479 |
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Target: 5'- uGCCCGCCACGAuGaauaaUUCGaCCUGCc -3' miRNA: 3'- gCGGGUGGUGCU-Cguc--AAGC-GGGCGc -5' |
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| 26840 | 3' | -60.7 | NC_005809.1 | + | 2367 | 0.73 | 0.131356 |
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Target: 5'- uCGgCCugCACGuGGCAGa-CGCCCGCGu -3' miRNA: 3'- -GCgGGugGUGC-UCGUCaaGCGGGCGC- -5' |
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| 26840 | 3' | -60.7 | NC_005809.1 | + | 2211 | 0.66 | 0.390893 |
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Target: 5'- aCGCCCucgucggguagcACCACGcgcAGCAGgcggCGCguggCCGCGc -3' miRNA: 3'- -GCGGG------------UGGUGC---UCGUCaa--GCG----GGCGC- -5' |
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| 26840 | 3' | -60.7 | NC_005809.1 | + | 402 | 0.67 | 0.309239 |
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Target: 5'- gCGCCCACCagcagcACG-GCGGUgaUCGUCgGCc -3' miRNA: 3'- -GCGGGUGG------UGCuCGUCA--AGCGGgCGc -5' |
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| 26840 | 3' | -60.7 | NC_005809.1 | + | 165 | 0.67 | 0.316788 |
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Target: 5'- uGCCCaagcuGCCGCGcGCAGaaUGCCgGCGu -3' miRNA: 3'- gCGGG-----UGGUGCuCGUCaaGCGGgCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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