Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26846 | 5' | -57.5 | NC_005809.1 | + | 28663 | 0.66 | 0.486363 |
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Target: 5'- aCAUCGcccugaACUGgaacaACCCCGACGCCa- -3' miRNA: 3'- cGUAGCcug---UGAC-----UGGGGCUGCGGau -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 21953 | 0.66 | 0.486363 |
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Target: 5'- cGCAgccGACACcGACCUgCGACGCCg- -3' miRNA: 3'- -CGUagcCUGUGaCUGGG-GCUGCGGau -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 18850 | 0.66 | 0.495659 |
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Target: 5'- cGCGccacCGaGGCACUGGCCCgCGACcugggcgcuaccgGCCUGu -3' miRNA: 3'- -CGUa---GC-CUGUGACUGGG-GCUG-------------CGGAU- -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 7433 | 0.66 | 0.507128 |
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Target: 5'- gGCGccCGGACACUu-CCUCGACGCg-- -3' miRNA: 3'- -CGUa-GCCUGUGAcuGGGGCUGCGgau -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 23320 | 0.66 | 0.507129 |
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Target: 5'- aGCAUC-GACGCcgagaaguucaUGGgCCUGACGCCg- -3' miRNA: 3'- -CGUAGcCUGUG-----------ACUgGGGCUGCGGau -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 26397 | 0.66 | 0.517652 |
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Target: 5'- gGCGUCGGugA---ACUCCGACGCg-- -3' miRNA: 3'- -CGUAGCCugUgacUGGGGCUGCGgau -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 34101 | 0.66 | 0.517652 |
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Target: 5'- cGCGUCGGcugcgGCAUcGcaggccACCUCGACGCCg- -3' miRNA: 3'- -CGUAGCC-----UGUGaC------UGGGGCUGCGGau -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 25111 | 0.66 | 0.528262 |
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Target: 5'- uGCAggcccuggCGuGGCAcCUGGCCUCGAUGCUg- -3' miRNA: 3'- -CGUa-------GC-CUGU-GACUGGGGCUGCGGau -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 20273 | 0.66 | 0.528262 |
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Target: 5'- aGCAUCGuGCuGCccGACUUCGACGCCg- -3' miRNA: 3'- -CGUAGCcUG-UGa-CUGGGGCUGCGGau -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 7627 | 0.66 | 0.538952 |
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Target: 5'- aGCGcCGGACACgUGGCCuuGGC-CUUGc -3' miRNA: 3'- -CGUaGCCUGUG-ACUGGggCUGcGGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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