Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26846 | 5' | -57.5 | NC_005809.1 | + | 30155 | 0.67 | 0.476132 |
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Target: 5'- cGCGUCGGACAUcagcGGCagUUGGCGCCa- -3' miRNA: 3'- -CGUAGCCUGUGa---CUGg-GGCUGCGGau -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 30407 | 1.08 | 0.000477 |
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Target: 5'- cGCAUCGGACACUGACCCCGACGCCUAc -3' miRNA: 3'- -CGUAGCCUGUGACUGGGGCUGCGGAU- -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 32960 | 0.68 | 0.39852 |
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Target: 5'- uGCGUCGauuacuGGCAa-GACCCgGACGCCa- -3' miRNA: 3'- -CGUAGC------CUGUgaCUGGGgCUGCGGau -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 34101 | 0.66 | 0.517652 |
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Target: 5'- cGCGUCGGcugcgGCAUcGcaggccACCUCGACGCCg- -3' miRNA: 3'- -CGUAGCC-----UGUGaC------UGGGGCUGCGGau -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 34977 | 0.67 | 0.466009 |
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Target: 5'- gGCAgaCGGACA--GACCCUGAC-CCUAc -3' miRNA: 3'- -CGUa-GCCUGUgaCUGGGGCUGcGGAU- -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 36036 | 0.68 | 0.407776 |
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Target: 5'- gGCGauUCGGGCgACgacGACCCCGAaaaacuCGCCa- -3' miRNA: 3'- -CGU--AGCCUG-UGa--CUGGGGCU------GCGGau -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 36278 | 0.7 | 0.291046 |
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Target: 5'- cGCAcCGGAgAUaaGCCCCGGCGCCc- -3' miRNA: 3'- -CGUaGCCUgUGacUGGGGCUGCGGau -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 36623 | 0.7 | 0.28379 |
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Target: 5'- cGCGcUGGACAaUGGCCCCGuguACGUCUGg -3' miRNA: 3'- -CGUaGCCUGUgACUGGGGC---UGCGGAU- -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 37475 | 0.75 | 0.141705 |
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Target: 5'- cGCAcCGGACGCUGGgCCgGcCGCCUGu -3' miRNA: 3'- -CGUaGCCUGUGACUgGGgCuGCGGAU- -5' |
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| 26846 | 5' | -57.5 | NC_005809.1 | + | 41929 | 0.67 | 0.446106 |
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Target: 5'- aGCcUUGGGCACcGA-CUCGACGCCg- -3' miRNA: 3'- -CGuAGCCUGUGaCUgGGGCUGCGGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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