Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26848 | 5' | -55.3 | NC_005809.1 | + | 13112 | 0.66 | 0.600889 |
Target: 5'- cGCGCGCUUCGuCGCCugguGCCaGgagcacCUGGg -3' miRNA: 3'- aUGCGUGAAGC-GUGGu---CGGaCa-----GACU- -5' |
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26848 | 5' | -55.3 | NC_005809.1 | + | 3848 | 0.66 | 0.589555 |
Target: 5'- aGCGCACUUUGCGcaccucgccgcCCAGCUUc-CUGAc -3' miRNA: 3'- aUGCGUGAAGCGU-----------GGUCGGAcaGACU- -5' |
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26848 | 5' | -55.3 | NC_005809.1 | + | 11940 | 0.68 | 0.4903 |
Target: 5'- cUGCGCcCUcauUCGCGCCGGCUgGUCg-- -3' miRNA: 3'- -AUGCGuGA---AGCGUGGUCGGaCAGacu -5' |
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26848 | 5' | -55.3 | NC_005809.1 | + | 40138 | 0.68 | 0.448673 |
Target: 5'- gGCGCcgauCUUCGagGCCGGCCUG-CUGc -3' miRNA: 3'- aUGCGu---GAAGCg-UGGUCGGACaGACu -5' |
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26848 | 5' | -55.3 | NC_005809.1 | + | 34810 | 0.7 | 0.380846 |
Target: 5'- gGCGCAgaUCGCGCCGGUgCUGcaaucgCUGAc -3' miRNA: 3'- aUGCGUgaAGCGUGGUCG-GACa-----GACU- -5' |
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26848 | 5' | -55.3 | NC_005809.1 | + | 29773 | 1.07 | 0.000765 |
Target: 5'- cUACGCACUUCGCACCAGCCUGUCUGAg -3' miRNA: 3'- -AUGCGUGAAGCGUGGUCGGACAGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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