Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2686 | 5' | -57.5 | NC_001491.2 | + | 77692 | 0.66 | 0.804884 |
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Target: 5'- uCAGaAGAUGUGUa-CGAGCuaUCCGCGg- -3' miRNA: 3'- -GUC-UCUACGCAgcGCUCG--AGGCGCaa -5' |
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| 2686 | 5' | -57.5 | NC_001491.2 | + | 105343 | 0.67 | 0.786848 |
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Target: 5'- aGGGGGUGCGUCuuuuggggGUGguuuGGCUCCGgGUg -3' miRNA: 3'- gUCUCUACGCAG--------CGC----UCGAGGCgCAa -5' |
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| 2686 | 5' | -57.5 | NC_001491.2 | + | 102769 | 0.67 | 0.768255 |
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Target: 5'- cCAGcguaaGGGUGCGUUGCGcGCUCCaGCa-- -3' miRNA: 3'- -GUC-----UCUACGCAGCGCuCGAGG-CGcaa -5' |
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| 2686 | 5' | -57.5 | NC_001491.2 | + | 131252 | 0.68 | 0.749179 |
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Target: 5'- cCGGAGG-GCGagacUCGCGAGggCCGUGUg -3' miRNA: 3'- -GUCUCUaCGC----AGCGCUCgaGGCGCAa -5' |
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| 2686 | 5' | -57.5 | NC_001491.2 | + | 114608 | 0.68 | 0.749179 |
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Target: 5'- cUAG-GAUGuUGUUgGUGAGCUCCGCGg- -3' miRNA: 3'- -GUCuCUAC-GCAG-CGCUCGAGGCGCaa -5' |
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| 2686 | 5' | -57.5 | NC_001491.2 | + | 131758 | 0.68 | 0.739482 |
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Target: 5'- --uGGAUGUGUCGCGuGCgauggCCGUGg- -3' miRNA: 3'- gucUCUACGCAGCGCuCGa----GGCGCaa -5' |
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| 2686 | 5' | -57.5 | NC_001491.2 | + | 12515 | 0.72 | 0.508113 |
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Target: 5'- gCGGGGcgGCGUCGCGgcGGCggCCGUGg- -3' miRNA: 3'- -GUCUCuaCGCAGCGC--UCGa-GGCGCaa -5' |
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| 2686 | 5' | -57.5 | NC_001491.2 | + | 52902 | 1.04 | 0.003797 |
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Target: 5'- cCAGAGAUGCGUCGCGAGCUCCGCGUUu -3' miRNA: 3'- -GUCUCUACGCAGCGCUCGAGGCGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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