Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26861 | 5' | -55.1 | NC_005809.1 | + | 32107 | 0.66 | 0.66808 |
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Target: 5'- gUGCUgCGCGaCUUCcaGGGCGAcgccaagGGCGc -3' miRNA: 3'- gACGA-GUGCaGAAG--CCCGCUua-----CCGC- -5' |
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| 26861 | 5' | -55.1 | NC_005809.1 | + | 13494 | 0.66 | 0.656915 |
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Target: 5'- aUGCcgacCGCGcCUUUGGGCGAGguaaGCGg -3' miRNA: 3'- gACGa---GUGCaGAAGCCCGCUUac--CGC- -5' |
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| 26861 | 5' | -55.1 | NC_005809.1 | + | 14962 | 0.66 | 0.645727 |
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Target: 5'- aUGCUCGCGUgaagUCGccGGUcgccaGAAUGGCGu -3' miRNA: 3'- gACGAGUGCAga--AGC--CCG-----CUUACCGC- -5' |
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| 26861 | 5' | -55.1 | NC_005809.1 | + | 18280 | 0.67 | 0.612127 |
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Target: 5'- uUGCcggC-CGUCagCGGGCcGGUGGCGa -3' miRNA: 3'- gACGa--GuGCAGaaGCCCGcUUACCGC- -5' |
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| 26861 | 5' | -55.1 | NC_005809.1 | + | 7775 | 0.68 | 0.545656 |
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Target: 5'- -gGCUCGCGcCUUCGcccugcgaGGCGAugGGCa -3' miRNA: 3'- gaCGAGUGCaGAAGC--------CCGCUuaCCGc -5' |
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| 26861 | 5' | -55.1 | NC_005809.1 | + | 31091 | 0.69 | 0.451058 |
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Target: 5'- gCUGCUCACcUCggugcaggCcGGCGAGUGGUGc -3' miRNA: 3'- -GACGAGUGcAGaa------GcCCGCUUACCGC- -5' |
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| 26861 | 5' | -55.1 | NC_005809.1 | + | 9324 | 0.7 | 0.402525 |
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Target: 5'- -gGCgCACGUCggCGcGGCGGGcGGCGg -3' miRNA: 3'- gaCGaGUGCAGaaGC-CCGCUUaCCGC- -5' |
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| 26861 | 5' | -55.1 | NC_005809.1 | + | 5416 | 0.71 | 0.384061 |
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Target: 5'- uCUGCgUACGcUCcaCGGcGCGGAUGGCGg -3' miRNA: 3'- -GACGaGUGC-AGaaGCC-CGCUUACCGC- -5' |
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| 26861 | 5' | -55.1 | NC_005809.1 | + | 22065 | 0.72 | 0.316013 |
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Target: 5'- uUGCUCGgGUCgcggUCGGcGCGcggcucGAUGGCGu -3' miRNA: 3'- gACGAGUgCAGa---AGCC-CGC------UUACCGC- -5' |
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| 26861 | 5' | -55.1 | NC_005809.1 | + | 26367 | 1.08 | 0.000913 |
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Target: 5'- gCUGCUCACGUCUUCGGGCGAAUGGCGc -3' miRNA: 3'- -GACGAGUGCAGAAGCCCGCUUACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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