Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
269 | 3' | -47 | AC_000008.1 | + | 14256 | 0.66 | 0.970473 |
Target: 5'- cCGUUugUGCC-UCcgCgguacCUGCGGCCu -3' miRNA: 3'- cGUAAugAUGGcAGuaGa----GAUGUCGG- -5' |
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269 | 3' | -47 | AC_000008.1 | + | 27085 | 0.72 | 0.745305 |
Target: 5'- cGCGaaaACUA-CGUCAUCUCcaGCGGCCa -3' miRNA: 3'- -CGUaa-UGAUgGCAGUAGAGa-UGUCGG- -5' |
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269 | 3' | -47 | AC_000008.1 | + | 4971 | 0.74 | 0.651296 |
Target: 5'- uGCA--GCUGCCGUCAUCcCUggcaggGgGGCCa -3' miRNA: 3'- -CGUaaUGAUGGCAGUAGaGA------UgUCGG- -5' |
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269 | 3' | -47 | AC_000008.1 | + | 26662 | 1.15 | 0.001878 |
Target: 5'- uGCAUUACUACCGUCAUCUCUACAGCCc -3' miRNA: 3'- -CGUAAUGAUGGCAGUAGAGAUGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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