Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2690 | 3' | -48.3 | NC_001491.2 | + | 144810 | 0.66 | 0.999337 |
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Target: 5'- cCCUCcg--CGgGGCucGGGAGUCGGAGAc -3' miRNA: 3'- -GGAGcuaaGCaCUG--UCUUCAGUCUCU- -5' |
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| 2690 | 3' | -48.3 | NC_001491.2 | + | 148981 | 0.67 | 0.998999 |
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Target: 5'- aCCUCGA-UCuUGAUGGGAGcgcggCGGAGGg -3' miRNA: 3'- -GGAGCUaAGcACUGUCUUCa----GUCUCU- -5' |
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| 2690 | 3' | -48.3 | NC_001491.2 | + | 93338 | 0.69 | 0.991225 |
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Target: 5'- cCCUUGAa-CGUGGCAGA-GUCAaGGGGc -3' miRNA: 3'- -GGAGCUaaGCACUGUCUuCAGU-CUCU- -5' |
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| 2690 | 3' | -48.3 | NC_001491.2 | + | 1460 | 0.71 | 0.97904 |
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Target: 5'- cCCUCGggUCucaUGGCGGcGAGUUGGAGAg -3' miRNA: 3'- -GGAGCuaAGc--ACUGUC-UUCAGUCUCU- -5' |
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| 2690 | 3' | -48.3 | NC_001491.2 | + | 100141 | 0.74 | 0.923811 |
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Target: 5'- cCCUCGA--CG-GACAGGAGggcUCGGAGAu -3' miRNA: 3'- -GGAGCUaaGCaCUGUCUUC---AGUCUCU- -5' |
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| 2690 | 3' | -48.3 | NC_001491.2 | + | 100251 | 1.12 | 0.01158 |
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Target: 5'- uCCUCGAUUCGUGACAGAAGUCAGAGAg -3' miRNA: 3'- -GGAGCUAAGCACUGUCUUCAGUCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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