Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2690 | 5' | -58.3 | NC_001491.2 | + | 109283 | 0.66 | 0.840476 |
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Target: 5'- aGCCCCCCgcagCCUUgcucguccugggcaUAUGACCGCuUCc -3' miRNA: 3'- aUGGGGGGa---GGAA--------------GUGCUGGUGcAGc -5' |
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| 2690 | 5' | -58.3 | NC_001491.2 | + | 115120 | 0.67 | 0.792158 |
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Target: 5'- gACCggggaCCUggCCUUCAcCGGCUGCGUCGa -3' miRNA: 3'- aUGGg----GGGa-GGAAGU-GCUGGUGCAGC- -5' |
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| 2690 | 5' | -58.3 | NC_001491.2 | + | 125121 | 0.67 | 0.783173 |
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Target: 5'- gGCCCCCa-CCUUCGCcgcaGCCGCGgcCGg -3' miRNA: 3'- aUGGGGGgaGGAAGUGc---UGGUGCa-GC- -5' |
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| 2690 | 5' | -58.3 | NC_001491.2 | + | 82668 | 0.67 | 0.75547 |
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Target: 5'- cACCUCCgCUCCgcgCugGGCCugGgCGu -3' miRNA: 3'- aUGGGGG-GAGGaa-GugCUGGugCaGC- -5' |
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| 2690 | 5' | -58.3 | NC_001491.2 | + | 8297 | 0.69 | 0.667637 |
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Target: 5'- aGCCCUCgUCUUUCccaGACUACGUUGa -3' miRNA: 3'- aUGGGGGgAGGAAGug-CUGGUGCAGC- -5' |
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| 2690 | 5' | -58.3 | NC_001491.2 | + | 137634 | 0.7 | 0.617468 |
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Target: 5'- -cCCCCCCUCCg--ACGGCCGcCGcCGc -3' miRNA: 3'- auGGGGGGAGGaagUGCUGGU-GCaGC- -5' |
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| 2690 | 5' | -58.3 | NC_001491.2 | + | 113033 | 0.7 | 0.607437 |
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Target: 5'- gACCCCCCcCCUUa--GACCACGcCc -3' miRNA: 3'- aUGGGGGGaGGAAgugCUGGUGCaGc -5' |
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| 2690 | 5' | -58.3 | NC_001491.2 | + | 116173 | 0.71 | 0.53808 |
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Target: 5'- aGCCCUUCUCCggcucCGCGGCCGCGg-- -3' miRNA: 3'- aUGGGGGGAGGaa---GUGCUGGUGCagc -5' |
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| 2690 | 5' | -58.3 | NC_001491.2 | + | 147059 | 0.72 | 0.480889 |
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Target: 5'- gGCUCCCCUCC---GCGGCCGCGgagcCGg -3' miRNA: 3'- aUGGGGGGAGGaagUGCUGGUGCa---GC- -5' |
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| 2690 | 5' | -58.3 | NC_001491.2 | + | 100214 | 1.07 | 0.002361 |
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Target: 5'- cUACCCCCCUCCUUCACGACCACGUCGg -3' miRNA: 3'- -AUGGGGGGAGGAAGUGCUGGUGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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