Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2694 | 5' | -50.8 | NC_001491.2 | + | 76201 | 0.66 | 0.990536 |
Target: 5'- uGCu-CUCUGUGCCUagacccCAUgccACGGCCgAg -3' miRNA: 3'- -CGuuGAGACACGGA------GUAa--UGCUGGgU- -5' |
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2694 | 5' | -50.8 | NC_001491.2 | + | 57272 | 0.68 | 0.966256 |
Target: 5'- uGCAugauGCUCUuugGUGCCUCGUugucaUugGGCCg- -3' miRNA: 3'- -CGU----UGAGA---CACGGAGUA-----AugCUGGgu -5' |
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2694 | 5' | -50.8 | NC_001491.2 | + | 57017 | 0.73 | 0.815722 |
Target: 5'- cGCAGCgc-GUccGUCUCGUUGCGACCCc -3' miRNA: 3'- -CGUUGagaCA--CGGAGUAAUGCUGGGu -5' |
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2694 | 5' | -50.8 | NC_001491.2 | + | 106283 | 1.12 | 0.005949 |
Target: 5'- cGCAACUCUGUGCCUCAUUACGACCCAc -3' miRNA: 3'- -CGUUGAGACACGGAGUAAUGCUGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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