Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26947 | 3' | -53.5 | NC_005809.1 | + | 8993 | 0.66 | 0.74506 |
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Target: 5'- gCGAUagccaGCGGUggCCGUGCCGgauuCGC-CAg -3' miRNA: 3'- -GCUAa----CGCCAaaGGUACGGC----GCGuGU- -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 21164 | 0.66 | 0.734204 |
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Target: 5'- uGAacGUGGaguucgUUUCCAUGCuCGCGCAg- -3' miRNA: 3'- gCUaaCGCC------AAAGGUACG-GCGCGUgu -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 16320 | 0.66 | 0.734204 |
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Target: 5'- aGGccgGCGGgugcCCGUGCCuucggcgacGCGCGCAa -3' miRNA: 3'- gCUaa-CGCCaaa-GGUACGG---------CGCGUGU- -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 18484 | 0.66 | 0.723239 |
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Target: 5'- ----aGCGGggugcCCGUGCCcCGCGCGg -3' miRNA: 3'- gcuaaCGCCaaa--GGUACGGcGCGUGU- -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 26291 | 0.67 | 0.644461 |
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Target: 5'- aCGAccG-GGUagccUUCCGggugGCCGCGCGCGa -3' miRNA: 3'- -GCUaaCgCCA----AAGGUa---CGGCGCGUGU- -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 9377 | 0.7 | 0.499049 |
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Target: 5'- gGAUUcgccaGCGGUggCCGUGCCGaggGCGCc -3' miRNA: 3'- gCUAA-----CGCCAaaGGUACGGCg--CGUGu -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 9257 | 0.72 | 0.361069 |
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Target: 5'- gGAUUcgccaGCGGUggCCGUGCCGCGauaGCc -3' miRNA: 3'- gCUAA-----CGCCAaaGGUACGGCGCg--UGu -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 9113 | 0.72 | 0.361069 |
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Target: 5'- gGAUUcgccaGCGGUggCCGUGCCGCGauaGCc -3' miRNA: 3'- gCUAA-----CGCCAaaGGUACGGCGCg--UGu -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 9305 | 0.66 | 0.74506 |
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Target: 5'- gGAUUcgccaGCGGUggCCGUGCCGUaauuGC-CAg -3' miRNA: 3'- gCUAA-----CGCCAaaGGUACGGCG----CGuGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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