Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26947 | 3' | -53.5 | NC_005809.1 | + | 24052 | 1.1 | 0.000911 |
Target: 5'- aCGAUUGCGGUUUCCAUGCCGCGCACAg -3' miRNA: 3'- -GCUAACGCCAAAGGUACGGCGCGUGU- -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 8665 | 0.76 | 0.220134 |
Target: 5'- ----cGCGGUcggcaUUgCGUGCCGCGCGCAc -3' miRNA: 3'- gcuaaCGCCA-----AAgGUACGGCGCGUGU- -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 9985 | 0.74 | 0.295037 |
Target: 5'- aCGGUgagcgugGCGGggUCgaaaGUGCCGCGCGCc -3' miRNA: 3'- -GCUAa------CGCCaaAGg---UACGGCGCGUGu -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 23914 | 0.73 | 0.343637 |
Target: 5'- ----cGCGGcUUCCAUGCCG-GCAUAg -3' miRNA: 3'- gcuaaCGCCaAAGGUACGGCgCGUGU- -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 9113 | 0.72 | 0.361069 |
Target: 5'- gGAUUcgccaGCGGUggCCGUGCCGCGauaGCc -3' miRNA: 3'- gCUAA-----CGCCAaaGGUACGGCGCg--UGu -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 9257 | 0.72 | 0.361069 |
Target: 5'- gGAUUcgccaGCGGUggCCGUGCCGCGauaGCc -3' miRNA: 3'- gCUAA-----CGCCAaaGGUACGGCGCg--UGu -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 11197 | 0.72 | 0.379108 |
Target: 5'- uGAUggUGCGGUca--AUGCCGCGCAUg -3' miRNA: 3'- gCUA--ACGCCAaaggUACGGCGCGUGu -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 28548 | 0.7 | 0.499049 |
Target: 5'- aCGAccUUGCGGccgccggCCAUcGCgGCGCGCAc -3' miRNA: 3'- -GCU--AACGCCaaa----GGUA-CGgCGCGUGU- -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 9377 | 0.7 | 0.499049 |
Target: 5'- gGAUUcgccaGCGGUggCCGUGCCGaggGCGCc -3' miRNA: 3'- gCUAA-----CGCCAaaGGUACGGCg--CGUGu -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 3126 | 0.69 | 0.520705 |
Target: 5'- aGcgUGCGGUUUCUgaAUGCcCGCgagaaaGCGCAa -3' miRNA: 3'- gCuaACGCCAAAGG--UACG-GCG------CGUGU- -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 1092 | 0.69 | 0.565031 |
Target: 5'- aGAUUGCcuuggCCGUGUCGCGCGgCAu -3' miRNA: 3'- gCUAACGccaaaGGUACGGCGCGU-GU- -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 7978 | 0.69 | 0.565031 |
Target: 5'- ----cGCaucgGGUUUCCAUGUCGcCGCGCu -3' miRNA: 3'- gcuaaCG----CCAAAGGUACGGC-GCGUGu -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 31164 | 0.68 | 0.610279 |
Target: 5'- uGGUUGCcuugUCCGgugaagcacggGCCGCGCACGu -3' miRNA: 3'- gCUAACGccaaAGGUa----------CGGCGCGUGU- -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 18514 | 0.68 | 0.610279 |
Target: 5'- ---cUGCGGUcaUCCAUGCCgGUGUugGg -3' miRNA: 3'- gcuaACGCCAa-AGGUACGG-CGCGugU- -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 23520 | 0.68 | 0.633064 |
Target: 5'- gCGAgcGCGccUUCCAgGCgGCGCACGc -3' miRNA: 3'- -GCUaaCGCcaAAGGUaCGgCGCGUGU- -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 26291 | 0.67 | 0.644461 |
Target: 5'- aCGAccG-GGUagccUUCCGggugGCCGCGCGCGa -3' miRNA: 3'- -GCUaaCgCCA----AAGGUa---CGGCGCGUGU- -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 40190 | 0.67 | 0.655846 |
Target: 5'- ----aGCGaccgCCAUGCCGUGCGCc -3' miRNA: 3'- gcuaaCGCcaaaGGUACGGCGCGUGu -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 28267 | 0.67 | 0.667206 |
Target: 5'- -uGUUGCGca--CCAUGaCCGCGCGCu -3' miRNA: 3'- gcUAACGCcaaaGGUAC-GGCGCGUGu -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 23720 | 0.66 | 0.701029 |
Target: 5'- aGGUcGCGGg--CCAgUGCCucggugGCGCGCAg -3' miRNA: 3'- gCUAaCGCCaaaGGU-ACGG------CGCGUGU- -5' |
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26947 | 3' | -53.5 | NC_005809.1 | + | 18484 | 0.66 | 0.723239 |
Target: 5'- ----aGCGGggugcCCGUGCCcCGCGCGg -3' miRNA: 3'- gcuaaCGCCaaa--GGUACGGcGCGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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