Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26947 | 3' | -53.5 | NC_005809.1 | + | 28548 | 0.7 | 0.499049 |
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Target: 5'- aCGAccUUGCGGccgccggCCAUcGCgGCGCGCAc -3' miRNA: 3'- -GCU--AACGCCaaa----GGUA-CGgCGCGUGU- -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 9377 | 0.7 | 0.499049 |
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Target: 5'- gGAUUcgccaGCGGUggCCGUGCCGaggGCGCc -3' miRNA: 3'- gCUAA-----CGCCAaaGGUACGGCg--CGUGu -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 11197 | 0.72 | 0.379108 |
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Target: 5'- uGAUggUGCGGUca--AUGCCGCGCAUg -3' miRNA: 3'- gCUA--ACGCCAaaggUACGGCGCGUGu -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 9257 | 0.72 | 0.361069 |
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Target: 5'- gGAUUcgccaGCGGUggCCGUGCCGCGauaGCc -3' miRNA: 3'- gCUAA-----CGCCAaaGGUACGGCGCg--UGu -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 9113 | 0.72 | 0.361069 |
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Target: 5'- gGAUUcgccaGCGGUggCCGUGCCGCGauaGCc -3' miRNA: 3'- gCUAA-----CGCCAaaGGUACGGCGCg--UGu -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 23914 | 0.73 | 0.343637 |
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Target: 5'- ----cGCGGcUUCCAUGCCG-GCAUAg -3' miRNA: 3'- gcuaaCGCCaAAGGUACGGCgCGUGU- -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 9985 | 0.74 | 0.295037 |
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Target: 5'- aCGGUgagcgugGCGGggUCgaaaGUGCCGCGCGCc -3' miRNA: 3'- -GCUAa------CGCCaaAGg---UACGGCGCGUGu -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 8665 | 0.76 | 0.220134 |
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Target: 5'- ----cGCGGUcggcaUUgCGUGCCGCGCGCAc -3' miRNA: 3'- gcuaaCGCCA-----AAgGUACGGCGCGUGU- -5' |
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| 26947 | 3' | -53.5 | NC_005809.1 | + | 24052 | 1.1 | 0.000911 |
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Target: 5'- aCGAUUGCGGUUUCCAUGCCGCGCACAg -3' miRNA: 3'- -GCUAACGCCAAAGGUACGGCGCGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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