Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26979 | 3' | -60.8 | NC_005809.1 | + | 7771 | 0.7 | 0.217328 |
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Target: 5'- aCCGGcgcGAUcuGCgCCGGCGUccacccuucgccgCCCAGCa -3' miRNA: 3'- -GGCCu--CUAuuCGgGGCCGCG-------------GGGUCG- -5' |
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| 26979 | 3' | -60.8 | NC_005809.1 | + | 3741 | 0.68 | 0.280354 |
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Target: 5'- gCCGGuGGUGGGCacgUCGGUGCCgCC-GCu -3' miRNA: 3'- -GGCCuCUAUUCGg--GGCCGCGG-GGuCG- -5' |
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| 26979 | 3' | -60.8 | NC_005809.1 | + | 30067 | 0.67 | 0.313562 |
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Target: 5'- gCCGGAaAUAuccuuugugcggcGCCCgGGCGCCaCGGCc -3' miRNA: 3'- -GGCCUcUAUu------------CGGGgCCGCGGgGUCG- -5' |
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| 26979 | 3' | -60.8 | NC_005809.1 | + | 167 | 0.67 | 0.324244 |
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Target: 5'- -aGGcGGAUGcGCCCCGGUGUCgCugGGCa -3' miRNA: 3'- ggCC-UCUAUuCGGGGCCGCGGgG--UCG- -5' |
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| 26979 | 3' | -60.8 | NC_005809.1 | + | 36202 | 0.67 | 0.348036 |
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Target: 5'- gCCGaccuGGUGuaaCCCaGCGCCCCGGCc -3' miRNA: 3'- -GGCcu--CUAUucgGGGcCGCGGGGUCG- -5' |
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| 26979 | 3' | -60.8 | NC_005809.1 | + | 34889 | 0.66 | 0.363737 |
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Target: 5'- gCCGccGAGGUcAAGCCCgacgaccUGGC-CCCCGGUg -3' miRNA: 3'- -GGC--CUCUA-UUCGGG-------GCCGcGGGGUCG- -5' |
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| 26979 | 3' | -60.8 | NC_005809.1 | + | 19032 | 0.66 | 0.364576 |
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Target: 5'- gCGGu----AGCCCUGGUagcgGCCgCCGGCg -3' miRNA: 3'- gGCCucuauUCGGGGCCG----CGG-GGUCG- -5' |
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| 26979 | 3' | -60.8 | NC_005809.1 | + | 36281 | 1.12 | 0.00014 |
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Target: 5'- aCCGGAGAUAAGCCCCGGCGCCCCAGCc -3' miRNA: 3'- -GGCCUCUAUUCGGGGCCGCGGGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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