Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26993 | 5' | -54.7 | NC_005809.1 | + | 5298 | 0.69 | 0.478781 |
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Target: 5'- -uGCAugucugGCAGGUCGAGGccgUAGGCcGCGa -3' miRNA: 3'- guCGU------UGUCUAGCUCCu--GUCCGaCGC- -5' |
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| 26993 | 5' | -54.7 | NC_005809.1 | + | 18111 | 0.69 | 0.457034 |
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Target: 5'- aAGCAGCAGGUCGucGGCgucaaugucgccgAGGCcGCGc -3' miRNA: 3'- gUCGUUGUCUAGCucCUG-------------UCCGaCGC- -5' |
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| 26993 | 5' | -54.7 | NC_005809.1 | + | 2966 | 0.7 | 0.40846 |
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Target: 5'- gAGC-ACGuGAUCGAuGACAGGUUGCu -3' miRNA: 3'- gUCGuUGU-CUAGCUcCUGUCCGACGc -5' |
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| 26993 | 5' | -54.7 | NC_005809.1 | + | 23906 | 0.7 | 0.389581 |
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Target: 5'- -uGUAACAGGcCGGGGGCGGGCgaccGCc -3' miRNA: 3'- guCGUUGUCUaGCUCCUGUCCGa---CGc -5' |
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| 26993 | 5' | -54.7 | NC_005809.1 | + | 12248 | 0.73 | 0.274201 |
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Target: 5'- gCGGCAucgaACGGAUCGGcgguaguguccuGGGCGGGUUGCu -3' miRNA: 3'- -GUCGU----UGUCUAGCU------------CCUGUCCGACGc -5' |
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| 26993 | 5' | -54.7 | NC_005809.1 | + | 2371 | 0.74 | 0.23381 |
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Target: 5'- aCGGCAACGGuUCGAGGcGCAcGGCaugGCGg -3' miRNA: 3'- -GUCGUUGUCuAGCUCC-UGU-CCGa--CGC- -5' |
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| 26993 | 5' | -54.7 | NC_005809.1 | + | 17830 | 0.74 | 0.227585 |
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Target: 5'- aCAGCAugGG-UCGAGGccgcGCAGGC-GCGg -3' miRNA: 3'- -GUCGUugUCuAGCUCC----UGUCCGaCGC- -5' |
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| 26993 | 5' | -54.7 | NC_005809.1 | + | 14761 | 0.78 | 0.130058 |
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Target: 5'- gGGCAGCGcGcgCGAGGACGuGCUGCGg -3' miRNA: 3'- gUCGUUGU-CuaGCUCCUGUcCGACGC- -5' |
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| 26993 | 5' | -54.7 | NC_005809.1 | + | 40231 | 1.08 | 0.000884 |
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Target: 5'- cCAGCAACAGAUCGAGGACAGGCUGCGc -3' miRNA: 3'- -GUCGUUGUCUAGCUCCUGUCCGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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