miRNA: 3'- -UAGUAG-----CUUGCaUAGCCgGGCUGCGc -5'
miRNA: 3'- -UAGUAGcUUGCa------UAGCCGGGCuGCGc -5'
miRNA: 3'- -UAGUAGCU--UGCAU-AGC-------CGgGCUGCGC- -5'
miRNA: 3'- -UAGUAGCUUGCauaGCCGGGcUGCGc -5'
miRNA: 3'- -UAGUAGCUUGCAUAGCCGGGCUGCGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26995 | 3' | -54.4 | NC_005809.1 | + | 24090 | 0.7 | 0.4883 |
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Target: 5'- gGUCAUCacgguGGACGaAUCGG-CCGACGCc -3' miRNA: 3'- -UAGUAG-----CUUGCaUAGCCgGGCUGCGc -5' |
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| 26995 | 3' | -54.4 | NC_005809.1 | + | 15238 | 0.7 | 0.477897 |
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Target: 5'- gAUCAUC-AACGgcagaggGUCGGCCUGcCGCa -3' miRNA: 3'- -UAGUAGcUUGCa------UAGCCGGGCuGCGc -5' |
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| 26995 | 3' | -54.4 | NC_005809.1 | + | 39614 | 0.7 | 0.44637 |
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Target: 5'- uGUCAUCGAucACGUGcUCGacgugccGCgCGACGCGg -3' miRNA: 3'- -UAGUAGCU--UGCAU-AGC-------CGgGCUGCGC- -5' |
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| 26995 | 3' | -54.4 | NC_005809.1 | + | 6089 | 0.72 | 0.354134 |
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Target: 5'- aGUCGUCGAGCG---CGGCCUuGCGCa -3' miRNA: 3'- -UAGUAGCUUGCauaGCCGGGcUGCGc -5' |
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| 26995 | 3' | -54.4 | NC_005809.1 | + | 40792 | 1.06 | 0.001548 |
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Target: 5'- aAUCAUCGAACGUAUCGGCCCGACGCGc -3' miRNA: 3'- -UAGUAGCUUGCAUAGCCGGGCUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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