Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27016 | 5' | -57.8 | NC_005832.1 | + | 92058 | 0.66 | 0.737282 |
Target: 5'- aGAGAcuccgcucACCCUGGAcuuugacgugGUGGacaaGGACUGCAa -3' miRNA: 3'- gUUCU--------UGGGGCCU----------CGCCg---UCUGACGU- -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 969 | 0.66 | 0.727271 |
Target: 5'- -uGGAACCUgGGGGa-GCAGACgUGCGc -3' miRNA: 3'- guUCUUGGGgCCUCgcCGUCUG-ACGU- -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 78667 | 0.66 | 0.727271 |
Target: 5'- uCAAGcccGCCCCaGAGUGGCcGGC-GCAg -3' miRNA: 3'- -GUUCu--UGGGGcCUCGCCGuCUGaCGU- -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 42162 | 0.66 | 0.707001 |
Target: 5'- uCAGGAGCgUCaacagGGAGacgaGGCAGACUGUc -3' miRNA: 3'- -GUUCUUGgGG-----CCUCg---CCGUCUGACGu -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 39783 | 0.67 | 0.686468 |
Target: 5'- aGAGGccCCCCGGAGCGGUGGGuccaaccggucCUaGCGg -3' miRNA: 3'- gUUCUu-GGGGCCUCGCCGUCU-----------GA-CGU- -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 87436 | 0.67 | 0.686468 |
Target: 5'- -uGGGGCCUCGGGGaUGGCGacggcGGCUGUg -3' miRNA: 3'- guUCUUGGGGCCUC-GCCGU-----CUGACGu -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 7948 | 0.67 | 0.65534 |
Target: 5'- aCGAG-ACCCUGGGGCuGGCGG-CgGCc -3' miRNA: 3'- -GUUCuUGGGGCCUCG-CCGUCuGaCGu -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 23177 | 0.67 | 0.65534 |
Target: 5'- --cGGAUCCCuGAGCcacGCAGACUGUg -3' miRNA: 3'- guuCUUGGGGcCUCGc--CGUCUGACGu -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 62304 | 0.67 | 0.65534 |
Target: 5'- uCGGGAGCCuuGGcGUaagaGGCAGAgUGCu -3' miRNA: 3'- -GUUCUUGGggCCuCG----CCGUCUgACGu -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 46177 | 0.67 | 0.65534 |
Target: 5'- aCAGGGACCCCuGGGCGGaccuGACUu-- -3' miRNA: 3'- -GUUCUUGGGGcCUCGCCgu--CUGAcgu -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 66907 | 0.67 | 0.644914 |
Target: 5'- aAAGAGCCaCCGGucccaucGUGGUAGACguacucggGCAg -3' miRNA: 3'- gUUCUUGG-GGCCu------CGCCGUCUGa-------CGU- -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 42420 | 0.68 | 0.634476 |
Target: 5'- cCGAGGACCCCGuGGCGGaucuGGCcaaGCAc -3' miRNA: 3'- -GUUCUUGGGGCcUCGCCgu--CUGa--CGU- -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 32427 | 0.68 | 0.612562 |
Target: 5'- aCGGGGACCCUGGAcagcgagugucccGUGGC-GACUGg- -3' miRNA: 3'- -GUUCUUGGGGCCU-------------CGCCGuCUGACgu -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 39280 | 0.68 | 0.603187 |
Target: 5'- aAGGGGCCCCGcGGGCG-CAGACg--- -3' miRNA: 3'- gUUCUUGGGGC-CUCGCcGUCUGacgu -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 36266 | 0.68 | 0.592791 |
Target: 5'- aGAGGACCCuCGcGGCGGCcu-CUGCGa -3' miRNA: 3'- gUUCUUGGG-GCcUCGCCGucuGACGU- -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 95513 | 0.69 | 0.572096 |
Target: 5'- uCGAGGugUCCGGAGCGGCcaggguccccgGGACa--- -3' miRNA: 3'- -GUUCUugGGGCCUCGCCG-----------UCUGacgu -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 53987 | 0.69 | 0.531289 |
Target: 5'- gCAAGAccuucaugGCCCUGGAGCuguggaggaGGCugGGGCUGCc -3' miRNA: 3'- -GUUCU--------UGGGGCCUCG---------CCG--UCUGACGu -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 5598 | 0.7 | 0.507311 |
Target: 5'- aCGGGGACCacaUGGAGaCGGCGGACggaucuccccuggGCAg -3' miRNA: 3'- -GUUCUUGGg--GCCUC-GCCGUCUGa------------CGU- -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 23419 | 0.7 | 0.501388 |
Target: 5'- gGAGGucGCCuUUGGAGCGcCAGACUGCGu -3' miRNA: 3'- gUUCU--UGG-GGCCUCGCcGUCUGACGU- -5' |
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27016 | 5' | -57.8 | NC_005832.1 | + | 95356 | 0.7 | 0.501388 |
Target: 5'- cCGAGGACCguaaagCCGGGGCGGUcuGGGuuCUGCGc -3' miRNA: 3'- -GUUCUUGG------GGCCUCGCCG--UCU--GACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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