Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27017 | 3' | -58.3 | NC_005832.1 | + | 54138 | 0.66 | 0.782959 |
Target: 5'- cGACGCCCUGCcCGagGaccUGUacgUGACGGg -3' miRNA: 3'- -CUGCGGGGCGuGCagU---ACGa--GCUGCC- -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 87658 | 0.66 | 0.754808 |
Target: 5'- uGAgGUCCCGCACGUCcUGacuuUCGcAgGGg -3' miRNA: 3'- -CUgCGGGGCGUGCAGuACg---AGC-UgCC- -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 1128 | 0.66 | 0.754808 |
Target: 5'- cGGCGUCCCGCGagGaCA-GgUCGGCGGc -3' miRNA: 3'- -CUGCGGGGCGUg-CaGUaCgAGCUGCC- -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 94300 | 0.66 | 0.745198 |
Target: 5'- --gGaCCUGCAUGUCAgGCUCGAUGu -3' miRNA: 3'- cugCgGGGCGUGCAGUaCGAGCUGCc -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 19971 | 0.67 | 0.705863 |
Target: 5'- --aGCCCgG-ACGUCAUGCa-GGCGGu -3' miRNA: 3'- cugCGGGgCgUGCAGUACGagCUGCC- -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 32515 | 0.67 | 0.705863 |
Target: 5'- uGAUGuCCuCCGUggGUCuuagGUGCUUGGCGGc -3' miRNA: 3'- -CUGC-GG-GGCGugCAG----UACGAGCUGCC- -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 44643 | 0.68 | 0.665536 |
Target: 5'- aGCGUCCUcUAC-UCggGCUCGACGGg -3' miRNA: 3'- cUGCGGGGcGUGcAGuaCGAGCUGCC- -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 65808 | 0.68 | 0.663504 |
Target: 5'- gGACGuCCCCGCGCc-CGUGCUggauccccugaaGACGGc -3' miRNA: 3'- -CUGC-GGGGCGUGcaGUACGAg-----------CUGCC- -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 4831 | 0.68 | 0.634977 |
Target: 5'- -uCGCCCCGUACucugCGUGCgacgcgUGugGGg -3' miRNA: 3'- cuGCGGGGCGUGca--GUACGa-----GCugCC- -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 2177 | 0.69 | 0.601346 |
Target: 5'- gGACGCCaCGgACGUCAUGgUccagcaaauugccaCGGCGGg -3' miRNA: 3'- -CUGCGGgGCgUGCAGUACgA--------------GCUGCC- -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 97176 | 0.69 | 0.594235 |
Target: 5'- gGACuCCCUGgACGUCAagaaGCUCGACaGGc -3' miRNA: 3'- -CUGcGGGGCgUGCAGUa---CGAGCUG-CC- -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 12886 | 0.69 | 0.553929 |
Target: 5'- cGGgGCCCUGCACGgCGUGgaCGugGu -3' miRNA: 3'- -CUgCGGGGCGUGCaGUACgaGCugCc -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 79015 | 0.77 | 0.222658 |
Target: 5'- uGACGCCUCuCACGUCAUGCagacCGACGa -3' miRNA: 3'- -CUGCGGGGcGUGCAGUACGa---GCUGCc -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 12656 | 0.77 | 0.217285 |
Target: 5'- -cCGCCCCGUccACGUCGUGCUUGGaGGc -3' miRNA: 3'- cuGCGGGGCG--UGCAGUACGAGCUgCC- -5' |
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27017 | 3' | -58.3 | NC_005832.1 | + | 1561 | 0.97 | 0.009246 |
Target: 5'- cGACGCCCCGCACGUCAUG-UCGACGGc -3' miRNA: 3'- -CUGCGGGGCGUGCAGUACgAGCUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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