Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27018 | 3' | -54.8 | NC_005832.1 | + | 17199 | 0.66 | 0.903642 |
Target: 5'- uGGCuuCUGGCaUGGuGUACUcg-CUCCa -3' miRNA: 3'- -CCGcuGACCG-ACCuCGUGAaaaGGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 100417 | 0.66 | 0.897057 |
Target: 5'- cGUucCUGGCcGGGGCGCUUUUUCg- -3' miRNA: 3'- cCGcuGACCGaCCUCGUGAAAAGGgg -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 95778 | 0.66 | 0.896385 |
Target: 5'- uGGCGGCUacccucgGGCUGGcuGCccucgGCUUgcugCCCUg -3' miRNA: 3'- -CCGCUGA-------CCGACCu-CG-----UGAAaa--GGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 11764 | 0.66 | 0.896385 |
Target: 5'- aGGuCGACgagGGCacccugcUGGA-CGCU-UUCCCCg -3' miRNA: 3'- -CC-GCUGa--CCG-------ACCUcGUGAaAAGGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 42432 | 0.66 | 0.868311 |
Target: 5'- uGGCGGauCUGGCca-AGCACg--UCCCUg -3' miRNA: 3'- -CCGCU--GACCGaccUCGUGaaaAGGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 22873 | 0.67 | 0.860547 |
Target: 5'- uGCGACgUGGCcauggaacaGGGGCAaauccUCCCCg -3' miRNA: 3'- cCGCUG-ACCGa--------CCUCGUgaaa-AGGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 9190 | 0.67 | 0.860547 |
Target: 5'- gGGa-GCUGGC-GGAGCAgga-UCCCCu -3' miRNA: 3'- -CCgcUGACCGaCCUCGUgaaaAGGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 29830 | 0.67 | 0.828245 |
Target: 5'- uGGCGGCguaGGCUGGAGacacagagcuagacUGCUUgaaccuccuguagCCCCu -3' miRNA: 3'- -CCGCUGa--CCGACCUC--------------GUGAAaa-----------GGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 55735 | 0.68 | 0.809628 |
Target: 5'- cGgGAgUGGCUGGGGCggcGCUgaccgCCUCu -3' miRNA: 3'- cCgCUgACCGACCUCG---UGAaaa--GGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 31159 | 0.68 | 0.800494 |
Target: 5'- uGGCGGCUGGaaGGAGgguggugccUGCUccgagCCCCa -3' miRNA: 3'- -CCGCUGACCgaCCUC---------GUGAaaa--GGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 26490 | 0.68 | 0.800494 |
Target: 5'- uGGaGACUGaCgGGGGCACggucaucgUCCCCg -3' miRNA: 3'- -CCgCUGACcGaCCUCGUGaaa-----AGGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 83422 | 0.7 | 0.681075 |
Target: 5'- cGGgGACauagaagagGGcCUGGAGCuCUUUggCCCCg -3' miRNA: 3'- -CCgCUGa--------CC-GACCUCGuGAAAa-GGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 64340 | 0.71 | 0.628506 |
Target: 5'- aGGCGGCcGGg-GGAGgACUgugCCCCu -3' miRNA: 3'- -CCGCUGaCCgaCCUCgUGAaaaGGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 9876 | 0.72 | 0.543912 |
Target: 5'- gGGCGgagggagGCaGGCUGGAGCugUcugcUCCCUa -3' miRNA: 3'- -CCGC-------UGaCCGACCUCGugAaa--AGGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 32399 | 0.75 | 0.436969 |
Target: 5'- uGGCGACUGGCUccGGGGCcCUaaaggCCUCc -3' miRNA: 3'- -CCGCUGACCGA--CCUCGuGAaaa--GGGG- -5' |
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27018 | 3' | -54.8 | NC_005832.1 | + | 1774 | 1.14 | 0.00111 |
Target: 5'- cGGCGACUGGCUGGAGCACUUUUCCCCu -3' miRNA: 3'- -CCGCUGACCGACCUCGUGAAAAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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