Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27018 | 5' | -57.6 | NC_005832.1 | + | 92542 | 0.74 | 0.31044 |
Target: 5'- gACGG-GGACGgcucuggGCUGCCUCCuccucGCGGGg -3' miRNA: 3'- -UGCCaCCUGU-------UGACGGAGGu----CGUCCg -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 75180 | 0.77 | 0.195393 |
Target: 5'- cACGGUGGACGcagacauguCUGCCUaCUGGCAGGg -3' miRNA: 3'- -UGCCACCUGUu--------GACGGA-GGUCGUCCg -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 1738 | 1.1 | 0.001018 |
Target: 5'- gACGGUGGACAACUGCCUCCAGCAGGCc -3' miRNA: 3'- -UGCCACCUGUUGACGGAGGUCGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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