Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27020 | 5' | -49.5 | NC_005832.1 | + | 50610 | 0.7 | 0.920897 |
Target: 5'- gCUGUCu---GCGGACAUUCUC-CUGa -3' miRNA: 3'- -GACAGuuugUGCCUGUGAGAGuGACg -5' |
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27020 | 5' | -49.5 | NC_005832.1 | + | 4347 | 0.72 | 0.872566 |
Target: 5'- -gGUCGAGCACGaGACG---UCGCUGCc -3' miRNA: 3'- gaCAGUUUGUGC-CUGUgagAGUGACG- -5' |
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27020 | 5' | -49.5 | NC_005832.1 | + | 62190 | 0.74 | 0.75328 |
Target: 5'- -aGUCAggUACGGGCAC-CUCACcaacGCc -3' miRNA: 3'- gaCAGUuuGUGCCUGUGaGAGUGa---CG- -5' |
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27020 | 5' | -49.5 | NC_005832.1 | + | 2591 | 0.99 | 0.035426 |
Target: 5'- gCUGUCAAACACGGACACUCcUACUGCu -3' miRNA: 3'- -GACAGUUUGUGCCUGUGAGaGUGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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