Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27021 | 3' | -54 | NC_005832.1 | + | 53203 | 0.66 | 0.906617 |
Target: 5'- ----gGCCCuGUAGGCCA--GGGCAg- -3' miRNA: 3'- caucaUGGG-CAUCCGGUagUCCGUag -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 54890 | 0.67 | 0.864024 |
Target: 5'- -aAGUACCUGUcgacguccgccgccAGGCaCAggccgguccucucgCAGGCGUCg -3' miRNA: 3'- caUCAUGGGCA--------------UCCG-GUa-------------GUCCGUAG- -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 102639 | 0.67 | 0.838353 |
Target: 5'- aUGGUcucgaGCUCGgAGGCCGcagUCAGGCAgUCg -3' miRNA: 3'- cAUCA-----UGGGCaUCCGGU---AGUCCGU-AG- -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 55942 | 0.68 | 0.792875 |
Target: 5'- aGUAGcUGCCCGUGucGCCGUCgAGGUAa- -3' miRNA: 3'- -CAUC-AUGGGCAUc-CGGUAG-UCCGUag -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 98035 | 0.68 | 0.792875 |
Target: 5'- -aAGacuauCCCGaAGGUCcUCAGGCGUCg -3' miRNA: 3'- caUCau---GGGCaUCCGGuAGUCCGUAG- -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 72789 | 0.68 | 0.792875 |
Target: 5'- -cGGgACCCuuUGGGCCGUCGGGCc-- -3' miRNA: 3'- caUCaUGGGc-AUCCGGUAGUCCGuag -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 87248 | 0.69 | 0.773494 |
Target: 5'- -gAGaGCCUGaGGGCCAUggucaAGGCGUCu -3' miRNA: 3'- caUCaUGGGCaUCCGGUAg----UCCGUAG- -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 26465 | 0.69 | 0.773494 |
Target: 5'- --cGUcCCCGgcgAGGCCAcCGGGCAg- -3' miRNA: 3'- cauCAuGGGCa--UCCGGUaGUCCGUag -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 56471 | 0.69 | 0.753555 |
Target: 5'- cGUGGUgagguccagGCCCG-AGGCCuu--GGCAUCu -3' miRNA: 3'- -CAUCA---------UGGGCaUCCGGuaguCCGUAG- -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 38651 | 0.7 | 0.722787 |
Target: 5'- -gAGUACCCGUGGGUCGUgaccgAGGaccuCAUCg -3' miRNA: 3'- caUCAUGGGCAUCCGGUAg----UCC----GUAG- -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 91258 | 0.7 | 0.712344 |
Target: 5'- ----aGCCUGUGGGCCuugguGGCGUCa -3' miRNA: 3'- caucaUGGGCAUCCGGuagu-CCGUAG- -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 43931 | 0.72 | 0.605463 |
Target: 5'- -aGGgcCCCGUGGGCCAUCu--CGUCg -3' miRNA: 3'- caUCauGGGCAUCCGGUAGuccGUAG- -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 89373 | 0.72 | 0.594744 |
Target: 5'- -cAGUcCCUGaAGGCCGUgUAGGCGUCg -3' miRNA: 3'- caUCAuGGGCaUCCGGUA-GUCCGUAG- -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 71158 | 0.74 | 0.460747 |
Target: 5'- -cAGuUugCCGgagAGGCCGUCAGGcCGUCc -3' miRNA: 3'- caUC-AugGGCa--UCCGGUAGUCC-GUAG- -5' |
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27021 | 3' | -54 | NC_005832.1 | + | 3357 | 1.09 | 0.0026 |
Target: 5'- aGUAGUACCCGUAGGCCAUCAGGCAUCc -3' miRNA: 3'- -CAUCAUGGGCAUCCGGUAGUCCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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