Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27022 | 3' | -51.5 | NC_005832.1 | + | 39796 | 0.66 | 0.970058 |
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Target: 5'- aGCGguGGGUccaaccgguccuaGCGGGGCGccu-GGAu -3' miRNA: 3'- gUGCguCUCA-------------UGCCCCGCuuuuUCUu -5' |
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| 27022 | 3' | -51.5 | NC_005832.1 | + | 351 | 0.66 | 0.961499 |
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Target: 5'- cCGCGCAGGGUAcauaccagcacugguCGGGGUaGGcguGGAGAGc -3' miRNA: 3'- -GUGCGUCUCAU---------------GCCCCG-CUu--UUUCUU- -5' |
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| 27022 | 3' | -51.5 | NC_005832.1 | + | 50904 | 0.66 | 0.951814 |
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Target: 5'- gGCGCGuGGGacccaGGGGCGAcAAGGGAg -3' miRNA: 3'- gUGCGU-CUCaug--CCCCGCUuUUUCUU- -5' |
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| 27022 | 3' | -51.5 | NC_005832.1 | + | 24442 | 0.67 | 0.947335 |
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Target: 5'- gACGcCGGAGgcaGGGGUaGGAAAGGAAc -3' miRNA: 3'- gUGC-GUCUCaugCCCCG-CUUUUUCUU- -5' |
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| 27022 | 3' | -51.5 | NC_005832.1 | + | 35575 | 0.68 | 0.908415 |
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Target: 5'- gACGCAGAGgcgcccacgGCGGGGgGAAc----- -3' miRNA: 3'- gUGCGUCUCa--------UGCCCCgCUUuuucuu -5' |
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| 27022 | 3' | -51.5 | NC_005832.1 | + | 58556 | 0.69 | 0.88025 |
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Target: 5'- -uCGCAGAuGUACGGGGUGu---AGGu -3' miRNA: 3'- guGCGUCU-CAUGCCCCGCuuuuUCUu -5' |
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| 27022 | 3' | -51.5 | NC_005832.1 | + | 40731 | 0.69 | 0.864635 |
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Target: 5'- gACGCGGGGUACGGGuccacCGggGuGGGu -3' miRNA: 3'- gUGCGUCUCAUGCCCc----GCuuUuUCUu -5' |
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| 27022 | 3' | -51.5 | NC_005832.1 | + | 21276 | 0.71 | 0.793265 |
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Target: 5'- -uCGCGGGGUACauaGGGGUGAucAGGAc -3' miRNA: 3'- guGCGUCUCAUG---CCCCGCUuuUUCUu -5' |
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| 27022 | 3' | -51.5 | NC_005832.1 | + | 88171 | 0.72 | 0.742951 |
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Target: 5'- --gGCAGAugACaGGGGCGAAGAGGAc -3' miRNA: 3'- gugCGUCUcaUG-CCCCGCUUUUUCUu -5' |
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| 27022 | 3' | -51.5 | NC_005832.1 | + | 4955 | 1.06 | 0.006796 |
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Target: 5'- gCACGCAGAGUACGGGGCGAAAAAGAAg -3' miRNA: 3'- -GUGCGUCUCAUGCCCCGCUUUUUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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