Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27022 | 5' | -55.8 | NC_005832.1 | + | 12664 | 0.66 | 0.855256 |
Target: 5'- --aUCCGccGCCGCCCCGUccACGUcgugcuuggaGGCa -3' miRNA: 3'- caaAGGU--UGGCGGGGCGucUGCA----------CUG- -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 90232 | 0.66 | 0.847143 |
Target: 5'- ---gCCcAUCGuCCCCGCAGACGccGAa -3' miRNA: 3'- caaaGGuUGGC-GGGGCGUCUGCa-CUg -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 36091 | 0.66 | 0.847143 |
Target: 5'- ----aCGACCGCCCuggCGCuGGCGUcGGCc -3' miRNA: 3'- caaagGUUGGCGGG---GCGuCUGCA-CUG- -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 87629 | 0.66 | 0.830306 |
Target: 5'- --gUCCAG--GCCCCGCAGgACGgGAUg -3' miRNA: 3'- caaAGGUUggCGGGGCGUC-UGCaCUG- -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 39566 | 0.66 | 0.830306 |
Target: 5'- ---cCCAGCCGCuCCCaCGGACccGACc -3' miRNA: 3'- caaaGGUUGGCG-GGGcGUCUGcaCUG- -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 34949 | 0.66 | 0.821598 |
Target: 5'- --gUCCGGCCaUCCCGgGGcacuCGUGGCa -3' miRNA: 3'- caaAGGUUGGcGGGGCgUCu---GCACUG- -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 54991 | 0.66 | 0.821598 |
Target: 5'- ---cCCGACgGCCacggagGUAGACGUGGCc -3' miRNA: 3'- caaaGGUUGgCGGgg----CGUCUGCACUG- -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 31277 | 0.66 | 0.821598 |
Target: 5'- -cUUCCAGCCGCCaCGUcggucaGGACGgGAg -3' miRNA: 3'- caAAGGUUGGCGGgGCG------UCUGCaCUg -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 58518 | 0.67 | 0.812707 |
Target: 5'- --gUCC-GCCGCCCgGCAGAUuaUGAa -3' miRNA: 3'- caaAGGuUGGCGGGgCGUCUGc-ACUg -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 46810 | 0.69 | 0.66392 |
Target: 5'- ---aCCGugUGCgCCUGCAGGCGccUGGCa -3' miRNA: 3'- caaaGGUugGCG-GGGCGUCUGC--ACUG- -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 65745 | 0.69 | 0.65342 |
Target: 5'- --gUCCAGgcCCGaCCUgGCGGACGUGGg -3' miRNA: 3'- caaAGGUU--GGC-GGGgCGUCUGCACUg -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 12373 | 0.7 | 0.632374 |
Target: 5'- cGUUUCCGucuGCCGUCUgGCAGAUG-GAa -3' miRNA: 3'- -CAAAGGU---UGGCGGGgCGUCUGCaCUg -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 75013 | 0.7 | 0.621847 |
Target: 5'- cUUUUCGGCCaggGCCCCGUAGAgG-GACc -3' miRNA: 3'- cAAAGGUUGG---CGGGGCGUCUgCaCUG- -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 39938 | 0.7 | 0.611327 |
Target: 5'- --aUCCAGgCGCCCCGCuaGGACcGguugGACc -3' miRNA: 3'- caaAGGUUgGCGGGGCG--UCUG-Ca---CUG- -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 7634 | 0.72 | 0.498315 |
Target: 5'- -cUUCaAGCUgaggauaaGUCCCGCGGACGUGGCg -3' miRNA: 3'- caAAGgUUGG--------CGGGGCGUCUGCACUG- -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 71527 | 0.72 | 0.497325 |
Target: 5'- -cUUCC-GCCGCCCUcguggcccugucuGCAGuCGUGGCa -3' miRNA: 3'- caAAGGuUGGCGGGG-------------CGUCuGCACUG- -5' |
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27022 | 5' | -55.8 | NC_005832.1 | + | 4917 | 1.1 | 0.001552 |
Target: 5'- cGUUUCCAACCGCCCCGCAGACGUGACa -3' miRNA: 3'- -CAAAGGUUGGCGGGGCGUCUGCACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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