Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27023 | 3' | -58.2 | NC_005832.1 | + | 12709 | 0.66 | 0.702614 |
Target: 5'- -gGCCCUCaagCUCAUCAagGCCcaugggUCCAu -3' miRNA: 3'- gaCGGGAGg--GAGUAGUagCGG------AGGUu -5' |
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27023 | 3' | -58.2 | NC_005832.1 | + | 68100 | 0.67 | 0.661046 |
Target: 5'- aCUGCCUgcgagCCCUCAggGUCG-CUCCu- -3' miRNA: 3'- -GACGGGa----GGGAGUagUAGCgGAGGuu -5' |
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27023 | 3' | -58.2 | NC_005832.1 | + | 72490 | 0.67 | 0.661046 |
Target: 5'- -gGCCCUCCCUac-CAaUGUCUCCGu -3' miRNA: 3'- gaCGGGAGGGAguaGUaGCGGAGGUu -5' |
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27023 | 3' | -58.2 | NC_005832.1 | + | 6619 | 0.67 | 0.619083 |
Target: 5'- --cCCCgUCCUCGUCAUCcgccgccuccGCCUCCAGg -3' miRNA: 3'- gacGGGaGGGAGUAGUAG----------CGGAGGUU- -5' |
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27023 | 3' | -58.2 | NC_005832.1 | + | 80162 | 0.67 | 0.608597 |
Target: 5'- -aGUCCugcgUCCC-CGUCAUgaCGCCUCCAu -3' miRNA: 3'- gaCGGG----AGGGaGUAGUA--GCGGAGGUu -5' |
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27023 | 3' | -58.2 | NC_005832.1 | + | 50965 | 0.67 | 0.608597 |
Target: 5'- aUGCCCUUgCCUC--CGUCGUCUCCc- -3' miRNA: 3'- gACGGGAG-GGAGuaGUAGCGGAGGuu -5' |
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27023 | 3' | -58.2 | NC_005832.1 | + | 79804 | 0.68 | 0.59813 |
Target: 5'- cCUGCCCUgCCUCc---UgGCCUCCGc -3' miRNA: 3'- -GACGGGAgGGAGuaguAgCGGAGGUu -5' |
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27023 | 3' | -58.2 | NC_005832.1 | + | 29751 | 0.68 | 0.587689 |
Target: 5'- gCUGCCgugUgCCUCAUCuUgGCCUCCAc -3' miRNA: 3'- -GACGGg--AgGGAGUAGuAgCGGAGGUu -5' |
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27023 | 3' | -58.2 | NC_005832.1 | + | 18883 | 0.68 | 0.556598 |
Target: 5'- -aGUCCUCUUcgucguggUCAUaGUCGCCUCCAAa -3' miRNA: 3'- gaCGGGAGGG--------AGUAgUAGCGGAGGUU- -5' |
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27023 | 3' | -58.2 | NC_005832.1 | + | 24642 | 0.69 | 0.505954 |
Target: 5'- -cGCUCUCCUUgGUCAU-GCCUCCc- -3' miRNA: 3'- gaCGGGAGGGAgUAGUAgCGGAGGuu -5' |
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27023 | 3' | -58.2 | NC_005832.1 | + | 52390 | 0.69 | 0.505954 |
Target: 5'- -gGCCacuaUCCCagaCAgCGUCGCCUCCAGg -3' miRNA: 3'- gaCGGg---AGGGa--GUaGUAGCGGAGGUU- -5' |
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27023 | 3' | -58.2 | NC_005832.1 | + | 20909 | 0.71 | 0.41949 |
Target: 5'- cCUGCCCUCCCUCAgccuaauUUGguggagCGUCUCCc- -3' miRNA: 3'- -GACGGGAGGGAGU-------AGUa-----GCGGAGGuu -5' |
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27023 | 3' | -58.2 | NC_005832.1 | + | 5641 | 1.06 | 0.001525 |
Target: 5'- cCUGCCCUCCCUCAUCAUCGCCUCCAAa -3' miRNA: 3'- -GACGGGAGGGAGUAGUAGCGGAGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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