Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27023 | 5' | -55.4 | NC_005832.1 | + | 53340 | 0.66 | 0.889235 |
Target: 5'- aCGUgugGAAGGcugcccuggccuacaGGGccaugGAuuucgagcCCAUGACGGGCu -3' miRNA: 3'- -GCAa--CUUCC---------------CCCa----CU--------GGUACUGCCCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 65288 | 0.66 | 0.880053 |
Target: 5'- aCGU---AGGGGGcacgggacccaacagGACCAUGGucaGGGCg -3' miRNA: 3'- -GCAacuUCCCCCa--------------CUGGUACUg--CCCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 100449 | 0.66 | 0.878605 |
Target: 5'- aCGUUGGAGGacgagccGGUGACCGUcccccugGACaGGUu -3' miRNA: 3'- -GCAACUUCCc------CCACUGGUA-------CUGcCCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 11029 | 0.66 | 0.864407 |
Target: 5'- uCGUUGGucauGGGGGUGcCCGUua-GGaGCa -3' miRNA: 3'- -GCAACUu---CCCCCACuGGUAcugCC-CG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 16770 | 0.66 | 0.856616 |
Target: 5'- gCGUUccuGGGGuUGACCAUGugGacuggGGCg -3' miRNA: 3'- -GCAAcuuCCCCcACUGGUACugC-----CCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 42303 | 0.66 | 0.856616 |
Target: 5'- ---cGAGGGaGGccGUGGCCAUGuguGGGCu -3' miRNA: 3'- gcaaCUUCC-CC--CACUGGUACug-CCCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 53260 | 0.66 | 0.856616 |
Target: 5'- -uUUGAuGGGGGUGACaCGUucaGGGUc -3' miRNA: 3'- gcAACUuCCCCCACUG-GUAcugCCCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 46429 | 0.67 | 0.848615 |
Target: 5'- uGUggGAcaAGGcGGUGGCCGUGGCcaaGGCg -3' miRNA: 3'- gCAa-CU--UCCcCCACUGGUACUGc--CCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 35308 | 0.67 | 0.814644 |
Target: 5'- ---aGAGGGaGGGaUGcCCGUGACuGGCu -3' miRNA: 3'- gcaaCUUCC-CCC-ACuGGUACUGcCCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 55743 | 0.68 | 0.796592 |
Target: 5'- uGUgGGAGcGGGaGUGGCUggGGCGGcGCu -3' miRNA: 3'- gCAaCUUC-CCC-CACUGGuaCUGCC-CG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 99832 | 0.69 | 0.739036 |
Target: 5'- ---gGucGGGGGUGAgauCCAUGACGuacugguuGGCa -3' miRNA: 3'- gcaaCuuCCCCCACU---GGUACUGC--------CCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 7845 | 0.69 | 0.729048 |
Target: 5'- ---gGAGGGuGGGUGGCgGUGACaaaGGGg -3' miRNA: 3'- gcaaCUUCC-CCCACUGgUACUG---CCCg -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 10344 | 0.69 | 0.729048 |
Target: 5'- ---gGAGGGcGGGUucCCAUGACaGGCu -3' miRNA: 3'- gcaaCUUCC-CCCAcuGGUACUGcCCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 2462 | 0.69 | 0.718972 |
Target: 5'- aCGUcuaUGAuGaGGGUGACC-UGACaGGGCu -3' miRNA: 3'- -GCA---ACUuCcCCCACUGGuACUG-CCCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 93826 | 0.72 | 0.543326 |
Target: 5'- gGUUGAuccgguuuGGGGGGauugGGCCcggGugGGGCc -3' miRNA: 3'- gCAACU--------UCCCCCa---CUGGua-CugCCCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 19631 | 0.74 | 0.445815 |
Target: 5'- cCGUUGAAGGuGGGaGACgAgcGGCGGGUg -3' miRNA: 3'- -GCAACUUCC-CCCaCUGgUa-CUGCCCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 12099 | 0.79 | 0.222831 |
Target: 5'- uGUUGGAGGGGGcgGGgUA-GACGGGCa -3' miRNA: 3'- gCAACUUCCCCCa-CUgGUaCUGCCCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 33091 | 0.81 | 0.191564 |
Target: 5'- uGUUGAccGGGGUGGCCGuccaguuccUGAUGGGCc -3' miRNA: 3'- gCAACUucCCCCACUGGU---------ACUGCCCG- -5' |
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27023 | 5' | -55.4 | NC_005832.1 | + | 5607 | 1.13 | 0.00129 |
Target: 5'- aCGUUGAAGGGGGUGACCAUGACGGGCa -3' miRNA: 3'- -GCAACUUCCCCCACUGGUACUGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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