miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27025 3' -56.2 NC_005832.1 + 67889 0.66 0.852687
Target:  5'- cCUcUCUCCUgaCGGGCCUaggcucggaCUCUGUCc-- -3'
miRNA:   3'- -GGcAGAGGA--GCCCGGA---------GAGACAGuuc -5'
27025 3' -56.2 NC_005832.1 + 64236 0.66 0.852687
Target:  5'- aCGacagCCUCGGcCCgaccggCUCUGUCAAGg -3'
miRNA:   3'- gGCaga-GGAGCCcGGa-----GAGACAGUUC- -5'
27025 3' -56.2 NC_005832.1 + 32566 0.66 0.836385
Target:  5'- aCUGcCUCCUCGcGGCCUCUggagcgGUCc-- -3'
miRNA:   3'- -GGCaGAGGAGC-CCGGAGAga----CAGuuc -5'
27025 3' -56.2 NC_005832.1 + 80004 0.67 0.783172
Target:  5'- gCCGcccUCUCCUC-GGCCUgcucCUCggcaGUCAGGg -3'
miRNA:   3'- -GGC---AGAGGAGcCCGGA----GAGa---CAGUUC- -5'
27025 3' -56.2 NC_005832.1 + 28075 0.68 0.734929
Target:  5'- gCCGUCUCCggCGGGaCC-CUUUGcCAc- -3'
miRNA:   3'- -GGCAGAGGa-GCCC-GGaGAGACaGUuc -5'
27025 3' -56.2 NC_005832.1 + 102363 0.7 0.643111
Target:  5'- uCUGUCUCCUCuucGGCCUCgg-GUCGc- -3'
miRNA:   3'- -GGCAGAGGAGc--CCGGAGagaCAGUuc -5'
27025 3' -56.2 NC_005832.1 + 39997 0.7 0.62238
Target:  5'- gUCGUCUCCcuUUGGGCC-CUCUGg---- -3'
miRNA:   3'- -GGCAGAGG--AGCCCGGaGAGACaguuc -5'
27025 3' -56.2 NC_005832.1 + 6551 0.97 0.014116
Target:  5'- aCCGUCUCCUCGGGCCUCU-UGUCAAGc -3'
miRNA:   3'- -GGCAGAGGAGCCCGGAGAgACAGUUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.