Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27026 | 5' | -57.2 | NC_005832.1 | + | 44690 | 0.66 | 0.779192 |
Target: 5'- cUCCCUCUcgaAGGC--GGUGG-CgUCGUAg -3' miRNA: 3'- -AGGGAGG---UCCGuuUCACCaGgAGCGU- -5' |
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27026 | 5' | -57.2 | NC_005832.1 | + | 40343 | 0.66 | 0.76674 |
Target: 5'- cUCCCUauuugCGGGCGccauagcccggcagGAcGUGGUUCUCGCc -3' miRNA: 3'- -AGGGAg----GUCCGU--------------UU-CACCAGGAGCGu -5' |
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27026 | 5' | -57.2 | NC_005832.1 | + | 37253 | 0.66 | 0.759946 |
Target: 5'- cUCCCacUCCcgaGGGCAu-GUGGUCCcUGCu -3' miRNA: 3'- -AGGG--AGG---UCCGUuuCACCAGGaGCGu -5' |
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27026 | 5' | -57.2 | NC_005832.1 | + | 52244 | 0.67 | 0.698654 |
Target: 5'- aCCCUCCuccccuggAGGCGAcgcugucugggauAGUGGccUCCaUCGCGu -3' miRNA: 3'- aGGGAGG--------UCCGUU-------------UCACC--AGG-AGCGU- -5' |
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27026 | 5' | -57.2 | NC_005832.1 | + | 5628 | 0.68 | 0.658186 |
Target: 5'- cUCCC-CUGGGCAGGGUGaccagacCCUCGUAc -3' miRNA: 3'- -AGGGaGGUCCGUUUCACca-----GGAGCGU- -5' |
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27026 | 5' | -57.2 | NC_005832.1 | + | 1736 | 0.68 | 0.647734 |
Target: 5'- -aCgUCCgAGGCGuaccuccuGUGGUCCUCGUg -3' miRNA: 3'- agGgAGG-UCCGUuu------CACCAGGAGCGu -5' |
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27026 | 5' | -57.2 | NC_005832.1 | + | 52371 | 0.68 | 0.647734 |
Target: 5'- gUCgCCUCCAGGgGAGGagGGUCgUgGCu -3' miRNA: 3'- -AG-GGAGGUCCgUUUCa-CCAGgAgCGu -5' |
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27026 | 5' | -57.2 | NC_005832.1 | + | 91549 | 0.7 | 0.533702 |
Target: 5'- aUCUUCCAGGCAAAG-GGUUCgUCGg- -3' miRNA: 3'- aGGGAGGUCCGUUUCaCCAGG-AGCgu -5' |
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27026 | 5' | -57.2 | NC_005832.1 | + | 54876 | 0.7 | 0.533702 |
Target: 5'- gUCCgccgCCAGGCAcAGgccGGUCCucUCGCAg -3' miRNA: 3'- aGGGa---GGUCCGUuUCa--CCAGG--AGCGU- -5' |
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27026 | 5' | -57.2 | NC_005832.1 | + | 49457 | 0.72 | 0.445973 |
Target: 5'- cUCCCUCaGGGCucguuGUGGUCCcagUCGUAg -3' miRNA: 3'- -AGGGAGgUCCGuuu--CACCAGG---AGCGU- -5' |
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27026 | 5' | -57.2 | NC_005832.1 | + | 7065 | 0.74 | 0.319791 |
Target: 5'- gUCCCUCacugugccgucgUAGGCAGAGaccaGGUCCUCGUu -3' miRNA: 3'- -AGGGAG------------GUCCGUUUCa---CCAGGAGCGu -5' |
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27026 | 5' | -57.2 | NC_005832.1 | + | 43202 | 0.75 | 0.284121 |
Target: 5'- cUCCCgCCAGGCuggcGUGGUCCggCGCu -3' miRNA: 3'- -AGGGaGGUCCGuuu-CACCAGGa-GCGu -5' |
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27026 | 5' | -57.2 | NC_005832.1 | + | 80826 | 0.77 | 0.222336 |
Target: 5'- cCCCUCCAgGGCccugGAGGggacggGGUCCUCGUAg -3' miRNA: 3'- aGGGAGGU-CCG----UUUCa-----CCAGGAGCGU- -5' |
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27026 | 5' | -57.2 | NC_005832.1 | + | 7377 | 1.08 | 0.001498 |
Target: 5'- cUCCCUCCAGGCAAAGUGGUCCUCGCAc -3' miRNA: 3'- -AGGGAGGUCCGUUUCACCAGGAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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