Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27029 | 5' | -57.6 | NC_005832.1 | + | 32115 | 0.66 | 0.758241 |
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Target: 5'- uAGGGcGGUcagccUCGCCCcCCUCACCaCGg -3' miRNA: 3'- -UUUCuCCA-----AGCGGGaGGAGUGGcGCa -5' |
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| 27029 | 5' | -57.6 | NC_005832.1 | + | 6049 | 0.66 | 0.71831 |
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Target: 5'- cGGGAGGUagaggUUGCCCUCgUCcCCGuCGa -3' miRNA: 3'- uUUCUCCA-----AGCGGGAGgAGuGGC-GCa -5' |
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| 27029 | 5' | -57.6 | NC_005832.1 | + | 50393 | 0.67 | 0.708109 |
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Target: 5'- cAGAGAGGUg-GCCCUCgaCGCC-CGa -3' miRNA: 3'- -UUUCUCCAagCGGGAGgaGUGGcGCa -5' |
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| 27029 | 5' | -57.6 | NC_005832.1 | + | 52359 | 0.68 | 0.603948 |
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Target: 5'- gGAGGAGGgUCGUggCUCCUgGCCGUGg -3' miRNA: 3'- -UUUCUCCaAGCGg-GAGGAgUGGCGCa -5' |
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| 27029 | 5' | -57.6 | NC_005832.1 | + | 11558 | 0.69 | 0.58207 |
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Target: 5'- --cGGGGUcgUCcuggacgGCCCUCCUCAUgGCGa -3' miRNA: 3'- uuuCUCCA--AG-------CGGGAGGAGUGgCGCa -5' |
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| 27029 | 5' | -57.6 | NC_005832.1 | + | 66285 | 0.69 | 0.572741 |
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Target: 5'- cAAGAGGUUCagggaaGCCUccauUCC-CGCCGCGg -3' miRNA: 3'- uUUCUCCAAG------CGGG----AGGaGUGGCGCa -5' |
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| 27029 | 5' | -57.6 | NC_005832.1 | + | 2041 | 0.83 | 0.081195 |
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Target: 5'- --uGGGGUcUGCCCUCCUCACCGUGg -3' miRNA: 3'- uuuCUCCAaGCGGGAGGAGUGGCGCa -5' |
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| 27029 | 5' | -57.6 | NC_005832.1 | + | 10559 | 1.04 | 0.002475 |
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Target: 5'- gAAAGAGGUUCGCCCUCCUCACCGCGUc -3' miRNA: 3'- -UUUCUCCAAGCGGGAGGAGUGGCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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