Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27030 | 3' | -55.5 | NC_005832.1 | + | 51039 | 0.66 | 0.87941 |
Target: 5'- aGGCAGGU-----GGGACCGacgggCAGGuGGg -3' miRNA: 3'- -UCGUCCAaagacUCCUGGCa----GUCC-CC- -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 19984 | 0.66 | 0.856603 |
Target: 5'- uGCAGGcggucCUGAGGAUCGcucccacCAGGGa -3' miRNA: 3'- uCGUCCaaa--GACUCCUGGCa------GUCCCc -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 86934 | 0.67 | 0.840325 |
Target: 5'- cGGUGGGg--UUGAGGAgcCCGUgAGGGu -3' miRNA: 3'- -UCGUCCaaaGACUCCU--GGCAgUCCCc -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 22750 | 0.67 | 0.840325 |
Target: 5'- uGCAuGGa----GAGGauaGCCGUCAGGGGu -3' miRNA: 3'- uCGU-CCaaagaCUCC---UGGCAGUCCCC- -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 19506 | 0.67 | 0.840325 |
Target: 5'- cAGCGGaGccgCagaGAGGGCCaUCAGGGGa -3' miRNA: 3'- -UCGUC-CaaaGa--CUCCUGGcAGUCCCC- -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 75113 | 0.67 | 0.823253 |
Target: 5'- uGGCGGGccaCUG-GGACgaCGUgAGGGGa -3' miRNA: 3'- -UCGUCCaaaGACuCCUG--GCAgUCCCC- -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 1472 | 0.67 | 0.796303 |
Target: 5'- uGUAGGggUUgccgUGAGGACCaagaGGGGGg -3' miRNA: 3'- uCGUCCaaAG----ACUCCUGGcag-UCCCC- -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 82855 | 0.68 | 0.767956 |
Target: 5'- uGCgAGGUugcguUUCUcaucGAGGACCGUCGGaGGc -3' miRNA: 3'- uCG-UCCA-----AAGA----CUCCUGGCAGUC-CCc -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 2662 | 0.69 | 0.68855 |
Target: 5'- gGGUGGGUguggaaggacacGAGG-CCGUCGGGGGc -3' miRNA: 3'- -UCGUCCAaaga--------CUCCuGGCAGUCCCC- -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 5676 | 0.7 | 0.635351 |
Target: 5'- cGguGG-UUCUGAGGAgCUGgaagaCGGGGGa -3' miRNA: 3'- uCguCCaAAGACUCCU-GGCa----GUCCCC- -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 54798 | 0.72 | 0.562417 |
Target: 5'- gGGCAGG---CUaAGGGCCGaCGGGGGg -3' miRNA: 3'- -UCGUCCaaaGAcUCCUGGCaGUCCCC- -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 19697 | 0.72 | 0.521694 |
Target: 5'- cGGCGGGUccUCcGAaaccggccaaGACCGUCAGGGGg -3' miRNA: 3'- -UCGUCCAa-AGaCUc---------CUGGCAGUCCCC- -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 79684 | 0.72 | 0.521694 |
Target: 5'- gAGCAGGUUggccCUGAGGcCCGgguaAGGGu -3' miRNA: 3'- -UCGUCCAAa---GACUCCuGGCag--UCCCc -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 29357 | 0.77 | 0.28954 |
Target: 5'- gGGUuugAGGacugUCUGAGGAUCaGUCAGGGGa -3' miRNA: 3'- -UCG---UCCaa--AGACUCCUGG-CAGUCCCC- -5' |
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27030 | 3' | -55.5 | NC_005832.1 | + | 11203 | 1.11 | 0.00157 |
Target: 5'- cAGCAGGUUUCUGAGGACCGUCAGGGGg -3' miRNA: 3'- -UCGUCCAAAGACUCCUGGCAGUCCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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