Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27031 | 3' | -56.9 | NC_005832.1 | + | 32262 | 0.66 | 0.792809 |
Target: 5'- gGAGGCCUUUaggGCCccGGAGCcaGUcGCCACg -3' miRNA: 3'- -CUCUGGAAG---CGGu-UCUCG--CAcCGGUG- -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 43196 | 0.66 | 0.792809 |
Target: 5'- aGGGCgCUccCGCCAGGcuGGCGUGGUCcgGCg -3' miRNA: 3'- cUCUG-GAa-GCGGUUC--UCGCACCGG--UG- -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 7518 | 0.66 | 0.784341 |
Target: 5'- aGGGACCUgguggggugcucguUCGUCAGGAGgGaccuccuggaccugcUGGCCGu -3' miRNA: 3'- -CUCUGGA--------------AGCGGUUCUCgC---------------ACCGGUg -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 36524 | 0.66 | 0.783392 |
Target: 5'- -cGGCgUaCGCCAGGGcCGggGGCCACa -3' miRNA: 3'- cuCUGgAaGCGGUUCUcGCa-CCGGUG- -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 44066 | 0.66 | 0.768031 |
Target: 5'- gGGGGCCcUCGUCAGGAGgGUagagggaaagagaaaGGCgGCc -3' miRNA: 3'- -CUCUGGaAGCGGUUCUCgCA---------------CCGgUG- -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 29915 | 0.66 | 0.764138 |
Target: 5'- aGAGGCCUggucuggggCGgCGGGGGCGgcgguggaGGCCAa -3' miRNA: 3'- -CUCUGGAa--------GCgGUUCUCGCa-------CCGGUg -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 91441 | 0.66 | 0.75432 |
Target: 5'- -cGGCCgucaUgGCCAAGcuGGaCGUGGCCAg -3' miRNA: 3'- cuCUGGa---AgCGGUUC--UC-GCACCGGUg -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 79144 | 0.67 | 0.73435 |
Target: 5'- uGAGGCCUaCGUCucccuGAGGGUGagGGCCAg -3' miRNA: 3'- -CUCUGGAaGCGG-----UUCUCGCa-CCGGUg -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 73020 | 0.67 | 0.703715 |
Target: 5'- aAGGCCccgacgCGCCAGGuAGCGUcGUCGCa -3' miRNA: 3'- cUCUGGaa----GCGGUUC-UCGCAcCGGUG- -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 90931 | 0.67 | 0.702683 |
Target: 5'- -cGACCUcaggagccucaucUCgGCCGAGGGCG-GGCCc- -3' miRNA: 3'- cuCUGGA-------------AG-CGGUUCUCGCaCCGGug -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 5098 | 0.67 | 0.693362 |
Target: 5'- cAGACCcaaCGCCAGGca-GUGGCCAg -3' miRNA: 3'- cUCUGGaa-GCGGUUCucgCACCGGUg -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 67065 | 0.68 | 0.672504 |
Target: 5'- aGAGGCCUgaccuggaCGCCGAG-GCccuggaGGCCACu -3' miRNA: 3'- -CUCUGGAa-------GCGGUUCuCGca----CCGGUG- -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 10033 | 0.68 | 0.672504 |
Target: 5'- ----gCUUCGUCGGGcGCGUcGGCCGCg -3' miRNA: 3'- cucugGAAGCGGUUCuCGCA-CCGGUG- -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 75388 | 0.69 | 0.615696 |
Target: 5'- uGGGACCUgguggacccaaaGUCGGGGGcCGUGGCuCACa -3' miRNA: 3'- -CUCUGGAag----------CGGUUCUC-GCACCG-GUG- -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 27568 | 0.71 | 0.467145 |
Target: 5'- cGAGGCCUUC-CUggGAGCGgugugcacGGCCGu -3' miRNA: 3'- -CUCUGGAAGcGGuuCUCGCa-------CCGGUg -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 47409 | 0.72 | 0.411461 |
Target: 5'- cGGGAuCCUUguugcUGcCCAGGAGCGUcccGGCCACa -3' miRNA: 3'- -CUCU-GGAA-----GC-GGUUCUCGCA---CCGGUG- -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 90151 | 0.73 | 0.351963 |
Target: 5'- uGAGGCgaaaaaUGCCAAGAGCuUGGCCACu -3' miRNA: 3'- -CUCUGgaa---GCGGUUCUCGcACCGGUG- -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 76410 | 0.74 | 0.320807 |
Target: 5'- aGGACCUgCGCCGGGAGgaccGGCCACa -3' miRNA: 3'- cUCUGGAaGCGGUUCUCgca-CCGGUG- -5' |
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27031 | 3' | -56.9 | NC_005832.1 | + | 11341 | 1.08 | 0.001451 |
Target: 5'- cGAGACCUUCGCCAAGAGCGUGGCCACc -3' miRNA: 3'- -CUCUGGAAGCGGUUCUCGCACCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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