Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27033 | 5' | -50.8 | NC_005832.1 | + | 42553 | 0.66 | 0.983525 |
Target: 5'- cUCCAaGGAGGACuacguuagGCaCAUCCugGCCGg -3' miRNA: 3'- cAGGUaUCUCUUGua------UG-GUAGG--CGGC- -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 27728 | 0.66 | 0.979145 |
Target: 5'- cGUCUAUGGAGuACucAUCGUCCacgGCCGu -3' miRNA: 3'- -CAGGUAUCUCuUGuaUGGUAGG---CGGC- -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 60727 | 0.66 | 0.979145 |
Target: 5'- -aCCAUGGGGGugAUAagauCCAUCCuCCu -3' miRNA: 3'- caGGUAUCUCUugUAU----GGUAGGcGGc -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 102206 | 0.66 | 0.979145 |
Target: 5'- cUCCAUGGcGGACGguguUACCGUCUccugaGCCa -3' miRNA: 3'- cAGGUAUCuCUUGU----AUGGUAGG-----CGGc -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 47475 | 0.66 | 0.973954 |
Target: 5'- aGUCCcucuugaugAUAGGGAGCAgguCCA-CUGCCa -3' miRNA: 3'- -CAGG---------UAUCUCUUGUau-GGUaGGCGGc -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 58609 | 0.67 | 0.971034 |
Target: 5'- -aCCAUGGAGGGCG-ACCAggagaugaCGCCc -3' miRNA: 3'- caGGUAUCUCUUGUaUGGUag------GCGGc -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 7135 | 0.67 | 0.969802 |
Target: 5'- cGUCCGaccagcaccucugAGGGucCAUGCCGUgcCCGCCGu -3' miRNA: 3'- -CAGGUa------------UCUCuuGUAUGGUA--GGCGGC- -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 98096 | 0.67 | 0.967885 |
Target: 5'- -gCUucgGGGGAACGUcuGCCGUCCcacGCCGa -3' miRNA: 3'- caGGua-UCUCUUGUA--UGGUAGG---CGGC- -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 42568 | 0.67 | 0.967557 |
Target: 5'- -gCCGUcaGGGACGUGCUuggccagAUCCGCCa -3' miRNA: 3'- caGGUAucUCUUGUAUGG-------UAGGCGGc -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 62289 | 0.67 | 0.957 |
Target: 5'- -cCCAUAGAGG----GCCAUCUGCuCGu -3' miRNA: 3'- caGGUAUCUCUuguaUGGUAGGCG-GC- -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 39455 | 0.68 | 0.948485 |
Target: 5'- cGUCCccggGGGGGGuCAUGCUAgacCCGCCa -3' miRNA: 3'- -CAGGua--UCUCUU-GUAUGGUa--GGCGGc -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 8454 | 0.68 | 0.947118 |
Target: 5'- cGUCagaGUAG-GAAUAguccacguacucucUGCCGUCCGCCu -3' miRNA: 3'- -CAGg--UAUCuCUUGU--------------AUGGUAGGCGGc -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 5742 | 0.69 | 0.916569 |
Target: 5'- -cCCAggGGAGAu----CCGUCCGCCGu -3' miRNA: 3'- caGGUa-UCUCUuguauGGUAGGCGGC- -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 87599 | 0.69 | 0.90378 |
Target: 5'- cGUCCAUccuGGAGAcgaggugggagACGUcgGCCuuugCCGCCGg -3' miRNA: 3'- -CAGGUA---UCUCU-----------UGUA--UGGua--GGCGGC- -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 43670 | 0.69 | 0.90378 |
Target: 5'- aGUCCAacaUGGGGaAGCAgGCCAUaggcuuccCCGCCGu -3' miRNA: 3'- -CAGGU---AUCUC-UUGUaUGGUA--------GGCGGC- -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 2134 | 0.71 | 0.859217 |
Target: 5'- aGUCCGaGGGGGACAgggccacgACCAUgaCGCCGu -3' miRNA: 3'- -CAGGUaUCUCUUGUa-------UGGUAg-GCGGC- -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 24702 | 0.72 | 0.806373 |
Target: 5'- -gCCAUGGAGAucaucGCAUACC-UCCaGCCc -3' miRNA: 3'- caGGUAUCUCU-----UGUAUGGuAGG-CGGc -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 93505 | 0.72 | 0.806373 |
Target: 5'- -cCCAUAGuagcugGGAGCGUugUGUCUGCCGc -3' miRNA: 3'- caGGUAUC------UCUUGUAugGUAGGCGGC- -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 9731 | 0.73 | 0.766454 |
Target: 5'- -aCCGUAGGGAGCAgacagcuccagccUGCCucccUCCGCCc -3' miRNA: 3'- caGGUAUCUCUUGU-------------AUGGu---AGGCGGc -5' |
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27033 | 5' | -50.8 | NC_005832.1 | + | 93659 | 0.74 | 0.68381 |
Target: 5'- -gCCGUAGAGA----ACCAUCCGUCGu -3' miRNA: 3'- caGGUAUCUCUuguaUGGUAGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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