Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27035 | 3' | -57.2 | NC_005832.1 | + | 11353 | 0.66 | 0.812663 |
Target: 5'- aCGAGCUCGuguaaaaaguuucUGAGGAGcGccgcucCCCUGGcCa -3' miRNA: 3'- -GCUCGAGU-------------ACUCCUC-Ca-----GGGACCuGc -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 32334 | 0.66 | 0.80465 |
Target: 5'- gGGGCUg--GAGGGGGaUCCUugUGGGCu -3' miRNA: 3'- gCUCGAguaCUCCUCC-AGGG--ACCUGc -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 39241 | 0.66 | 0.80465 |
Target: 5'- -aGGCUCGggaucGGGGAccgugaaccuggGGUCCC-GGACGg -3' miRNA: 3'- gcUCGAGUa----CUCCU------------CCAGGGaCCUGC- -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 66706 | 0.66 | 0.795595 |
Target: 5'- cCGGGaC-CGUGuuuGGGGaccuGUCCCUGGACa -3' miRNA: 3'- -GCUC-GaGUACu--CCUC----CAGGGACCUGc -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 59899 | 0.66 | 0.786389 |
Target: 5'- uGAGaUCcuggAUGAGGAGG-CUCUGGAgGg -3' miRNA: 3'- gCUCgAG----UACUCCUCCaGGGACCUgC- -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 11780 | 0.66 | 0.777982 |
Target: 5'- -uGGCUCGgccggGGGGAGGUCgacgagggcacccugCUGGACGc -3' miRNA: 3'- gcUCGAGUa----CUCCUCCAGg--------------GACCUGC- -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 56547 | 0.66 | 0.767561 |
Target: 5'- uGGGCgcuccCGUGGGaguGGGGUUCCUGGcCGg -3' miRNA: 3'- gCUCGa----GUACUC---CUCCAGGGACCuGC- -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 92603 | 0.67 | 0.745303 |
Target: 5'- aGAGaaaaguacaaacucCUCAccAGGAGGcugUCCCUGGACa -3' miRNA: 3'- gCUC--------------GAGUacUCCUCC---AGGGACCUGc -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 33623 | 0.67 | 0.728501 |
Target: 5'- aGAGUUUucUGAGaaAGG-CCCUGGACGa -3' miRNA: 3'- gCUCGAGu-ACUCc-UCCaGGGACCUGC- -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 75757 | 0.68 | 0.702342 |
Target: 5'- aGGGC-CGUGAGGuccgcugcgggcgagAGGUCCauugguCUGGGCGc -3' miRNA: 3'- gCUCGaGUACUCC---------------UCCAGG------GACCUGC- -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 72567 | 0.68 | 0.69726 |
Target: 5'- uGGGgaCGUGGGGccccugggcuucaGGGUCCCgacgucguagUGGACGg -3' miRNA: 3'- gCUCgaGUACUCC-------------UCCAGGG----------ACCUGC- -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 10925 | 0.68 | 0.688077 |
Target: 5'- uCGGGCU--UGAGGAGGUCUCccgaguUGGAg- -3' miRNA: 3'- -GCUCGAguACUCCUCCAGGG------ACCUgc -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 56481 | 0.68 | 0.657228 |
Target: 5'- uGGGuCUUAUagGAGGAGGagcaguggCCCUGGGCu -3' miRNA: 3'- gCUC-GAGUA--CUCCUCCa-------GGGACCUGc -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 105021 | 0.69 | 0.626192 |
Target: 5'- aGAcGC-CAUGAGGAGGUaCCaGGGCa -3' miRNA: 3'- gCU-CGaGUACUCCUCCAgGGaCCUGc -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 11698 | 0.69 | 0.595197 |
Target: 5'- aCGucGC-CAUGAGGAGGgccgUCCaGGACGa -3' miRNA: 3'- -GCu-CGaGUACUCCUCCa---GGGaCCUGC- -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 46198 | 0.69 | 0.595197 |
Target: 5'- -cGGCUCAggcccuccugGAGGAcaGGgacCCCUGGGCGg -3' miRNA: 3'- gcUCGAGUa---------CUCCU--CCa--GGGACCUGC- -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 42516 | 0.7 | 0.574659 |
Target: 5'- gCGugcuGCUcCAUGAGGAGGgccgccaugCUCUGGugGu -3' miRNA: 3'- -GCu---CGA-GUACUCCUCCa--------GGGACCugC- -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 32486 | 0.7 | 0.554287 |
Target: 5'- -cAGCUCAUGuuGGAGGgCCCUacgcGGGCGc -3' miRNA: 3'- gcUCGAGUACu-CCUCCaGGGA----CCUGC- -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 79116 | 0.71 | 0.514248 |
Target: 5'- --cGCUUAcGAGGAGGUCCgUgaGGACGu -3' miRNA: 3'- gcuCGAGUaCUCCUCCAGGgA--CCUGC- -5' |
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27035 | 3' | -57.2 | NC_005832.1 | + | 12542 | 0.71 | 0.51129 |
Target: 5'- cCGAGCUCAUcAGGAGuguguuccucccgaGUUUCUGGACa -3' miRNA: 3'- -GCUCGAGUAcUCCUC--------------CAGGGACCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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