Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27035 | 5' | -60.3 | NC_005832.1 | + | 52929 | 0.71 | 0.419462 |
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Target: 5'- gCCCCUGgGCAGcAUGgCCCCggGACGcaAGGg -3' miRNA: 3'- -GGGGAUgCGUC-UGCgGGGG--CUGC--UCC- -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 19857 | 0.7 | 0.472602 |
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Target: 5'- gCCCCUGgGCcuGACGgucCCCCUGACGGu- -3' miRNA: 3'- -GGGGAUgCGu-CUGC---GGGGGCUGCUcc -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 36406 | 0.69 | 0.481789 |
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Target: 5'- gUCCauaguCGCAGAgGCCgCCG-CGAGGg -3' miRNA: 3'- -GGGgau--GCGUCUgCGGgGGCuGCUCC- -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 94233 | 0.69 | 0.481789 |
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Target: 5'- cCCCCUAUuuuCGGGCuucGCCCUCGACGGuucGGg -3' miRNA: 3'- -GGGGAUGc--GUCUG---CGGGGGCUGCU---CC- -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 86464 | 0.69 | 0.481789 |
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Target: 5'- aUCCUGCGCAGcagcaagaggaGCaGCCgCCUGAgGAGGc -3' miRNA: 3'- gGGGAUGCGUC-----------UG-CGG-GGGCUgCUCC- -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 32967 | 0.69 | 0.525092 |
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Target: 5'- uCUCCUGCcuucgGCAGACaccuccagccucuCCCCgGACGAGGc -3' miRNA: 3'- -GGGGAUG-----CGUCUGc------------GGGGgCUGCUCC- -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 32874 | 0.69 | 0.525092 |
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Target: 5'- uCUCCUGCcucccGCAGACaccuccagccucuCCCCgGACGAGGc -3' miRNA: 3'- -GGGGAUG-----CGUCUGc------------GGGGgCUGCUCC- -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 12992 | 1.1 | 0.000837 |
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Target: 5'- uCCCCUACGCAGACGCCCCCGACGAGGc -3' miRNA: 3'- -GGGGAUGCGUCUGCGGGGGCUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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