Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27038 | 5' | -52.6 | NC_005832.1 | + | 71710 | 0.66 | 0.939447 |
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Target: 5'- aUGGGUUU---CCAGGACucAGAGUUa -3' miRNA: 3'- gACCCAAAgacGGUCCUGu-UCUCAGa -5' |
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| 27038 | 5' | -52.6 | NC_005832.1 | + | 55270 | 0.66 | 0.928925 |
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Target: 5'- -aGGGUcuuuaggagcucUUCUGCagacuuGGGACAcgaAGAGUCg -3' miRNA: 3'- gaCCCA------------AAGACGg-----UCCUGU---UCUCAGa -5' |
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| 27038 | 5' | -52.6 | NC_005832.1 | + | 78254 | 0.67 | 0.917334 |
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Target: 5'- aCUGGGUUcCagGCCAGGAaccuggGAGAG-CUa -3' miRNA: 3'- -GACCCAAaGa-CGGUCCUg-----UUCUCaGA- -5' |
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| 27038 | 5' | -52.6 | NC_005832.1 | + | 48784 | 0.67 | 0.911139 |
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Target: 5'- -aGGGUgUCUGCCcgagAGuGGCAAGAGa-- -3' miRNA: 3'- gaCCCAaAGACGG----UC-CUGUUCUCaga -5' |
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| 27038 | 5' | -52.6 | NC_005832.1 | + | 98352 | 0.67 | 0.904679 |
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Target: 5'- -cGGGg--CUGCCaugaucaccaGGGACAGGAGg-- -3' miRNA: 3'- gaCCCaaaGACGG----------UCCUGUUCUCaga -5' |
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| 27038 | 5' | -52.6 | NC_005832.1 | + | 12773 | 0.67 | 0.897958 |
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Target: 5'- -aGGGcccCgcacGCCAGGGCGucAGAGUCUa -3' miRNA: 3'- gaCCCaaaGa---CGGUCCUGU--UCUCAGA- -5' |
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| 27038 | 5' | -52.6 | NC_005832.1 | + | 37419 | 0.68 | 0.876261 |
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Target: 5'- uUGGGagcCUGuCCGGGAUucucAGAGUCUa -3' miRNA: 3'- gACCCaaaGAC-GGUCCUGu---UCUCAGA- -5' |
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| 27038 | 5' | -52.6 | NC_005832.1 | + | 79163 | 0.68 | 0.868534 |
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Target: 5'- -aGGGUgaggGCCAGGgACAggaagGGAGUCg -3' miRNA: 3'- gaCCCAaagaCGGUCC-UGU-----UCUCAGa -5' |
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| 27038 | 5' | -52.6 | NC_005832.1 | + | 95332 | 0.7 | 0.769635 |
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Target: 5'- uCUGGG-UUCUgcGCCAcGGACAAGGGa-- -3' miRNA: 3'- -GACCCaAAGA--CGGU-CCUGUUCUCaga -5' |
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| 27038 | 5' | -52.6 | NC_005832.1 | + | 14249 | 1.08 | 0.004457 |
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Target: 5'- cCUGGGUUUCUGCCAGGACAAGAGUCUc -3' miRNA: 3'- -GACCCAAAGACGGUCCUGUUCUCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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